大学化学 >> 2018, Vol. 33 >> Issue (3): 48-54.doi: 10.3866/PKU.DXHX201709018

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生物正交反应法制备含有赖氨酸翻译后修饰类似物的蛋白质

王志鹏1,2,*(),程农壹4,李曼1,3,王田5   

  1. 1 Department of Chemistry, Texas A&M University, College Station, Texas 77840, USA
    2 清华大学化学系, 北京 100084
    3 Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77840, USA
    4 Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
    5 Division of Biological Science, University of California, San Diego, La Jolla 92093, USA
  • 收稿日期:2017-09-12 发布日期:2018-03-13
  • 通讯作者: 王志鹏 E-mail:zhipeng.wang@chem.tamu.edu

Applying Bioorthogonal Reactions to the Synthesis of Proteins Containing Lysine Post-Translational Modification Mimics

Zhipeng A. WANG1,2,*(),Nongyi CHENG4,Man LI1,3,Tian WANG5   

  1. 1 Department of Chemistry, Texas A & M University, College Station, Texas 77840, USA
    2 Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China
    3 Department of Plant Pathology and Microbiology, Texas A & M University, College Station, Texas 77840, USA
    4 Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA 53706
    5 Division of Biological Science, University of California, San Diego, La Jolla 92093, USA
  • Received:2017-09-12 Published:2018-03-13
  • Contact: Zhipeng A. WANG E-mail:zhipeng.wang@chem.tamu.edu

摘要:

翻译后修饰一直是表观遗传学的重要研究内容,尤其是近年来多种新型天然蛋白质中翻译后修饰被发现广泛存在于蛋白质组中。细胞生物学证明这些翻译后修饰对染色体结构和基因转录功能有关,但是其中具体的分子生物学机制还处于未知状态。为了后续的进一步研究,人们需要发展制备方法以求获取足量具有特定翻译后修饰的蛋白质。本文将讨论利用生物正交反应的手段制备含有这些新型赖氨酸翻译后修饰的蛋白的探索,期对教学与科研有助。

关键词: 翻译后修饰, 生物正交反应, 蛋白质组

Abstract:

As an important topic in epigenetic research, post-translational modifications (PTMs) have been drawing more and more attention recently. With the increasing number of novel PTMs discovered, they have been found widely existing in human proteome. Although cell biology research showed their significant roles in the regulation of chromatin structure and gene transcription functions, the more detailed functions are remaining unclear. For further biology research, chemical biologists need to develop synthetic strategies for the proteins with specific PTMs at desired sites. This manuscript will introduce the application of bioorthogonal reactions to protein chemistry for the incorporation of lysine PTMs. We hope it can contribute to the research and education ongoing.

Key words: Post-translational modification, Bioorthogonal reaction, Proteome

MSC2000: 

  • G64