大学化学 >> 2018, Vol. 33 >> Issue (12): 67-70.doi: 10.3866/PKU.DXHX201804029

化学实验 上一篇    下一篇

构树叶中黄酮类化合物的提取及测定——介绍一个大学综合化学实验

陈媛梅*(),丁来欣   

  • 收稿日期:2018-04-24 发布日期:2018-12-14
  • 通讯作者: 陈媛梅 E-mail:chym11@bjfu.edu.cn
  • 基金资助:
    中央高校基本科研业务费专项资金(2015ZCQ-LY-03)

Extraction and Determination of Flavonoids from Broussonetia Papyrifera (L.) Vent. Leaves: A Comprehensive Chemical Experiment for College Students

Yuanmei CHEN*(),Laixin DING   

  • Received:2018-04-24 Published:2018-12-14
  • Contact: Yuanmei CHEN E-mail:chym11@bjfu.edu.cn
  • Supported by:
    中央高校基本科研业务费专项资金(2015ZCQ-LY-03)

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摘要：

介绍一个大学综合化学实验。该实验是一个科研转化的大学生综合化学实验，内容包括芦丁吸收曲线和标准曲线的绘制、超声波辅助溶剂提取构树叶中的黄酮并用分光光度法测定总黄酮。该实验分析植物样品中的有效成分，并综合运用多种定量分析基本操作，满足农林院校相关专业对化学实验的特殊需求，有利于训练学生的综合实验技能，提高学生创新能力，可作为非化学专业低年级分析化学实验课的综合实验。

关键词: 构树, 黄酮, 超声波提取, 分光光度法, 综合化学实验

Abstract:

A new comprehensive chemical experiment for college students is introduced. This experiment is from the achievements of scientific research. It includes obtaining absorption spectrum and calibration curve of Rutin, and ultrasonic-assisted ethanol extraction and spectrophotometric determination of flavonoids from Broussonetia papyrifera (L.) Vent. Leaves. The experiment involves several basic analytical chemistry procedures and the effective components in plant sample are analyzed. Through the experiment, the students' experimental skills, comprehensive and innovative abilities are improved; furthermore, the specialty requirements are satisfied in agriculture or forestry universities. Thus, it is recommended to be included in the comprehensive analytical chemistry laboratory for students of non-chemistry majors.

Key words: Broussonetia papyrifera (L.) Vent., Flavonoids, Utrasonic-assisted extraction, UV-Vis spectroscopy, Comprehensive chemical experiment

MSC2000: 

  • G64