大学化学 >> 2019, Vol. 34 >> Issue (3): 36-41.doi: 10.3866/PKU.DXHX201807034

化学实验 上一篇    下一篇

两种比色法检测水体中微量氨含量的比较研究

王佳敏1,李涵1,张文凯2,刘承上3,刘宝良1,孙华宇1,张其坤1,*()   

  1. 1 山东师范大学化学化工与材料科学学院, 化学国家级实验教学示范中心, 济南 250014
    2 邯郸市第一中学, 河北 邯郸 056001
    3 山东师范大学附属中学, 济南 250014
  • 收稿日期:2018-07-26 录用日期:2018-08-19 发布日期:2019-03-09
  • 通讯作者: 张其坤 E-mail:zhangqk@sdnu.edu.cn
  • 基金资助:
    山东师范大学本科生科研基金项目(2017BKSKY49);山东师范大学本科生科研基金项目(2018BKSKY54);大学生创新训练计划项目(20181501);山东师范大学教学改革项目:工程教育认证背景下化学工程与工艺专业建设与教学改革新探索

Comparison of Two Colorimetric Methods for the Determination of Trace Ammonia in Water

Jiamin WANG1,Han LI1,Wenkai ZHANG2,Chengshang LIU3,Baoliang LIU1,Huayu SUN1,Qikun ZHANG1,*()   

  1. 1 Chemical National Experimental Teaching Demonstration Center, College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Ji'nan 250014, P. R. China
    2 Handan No.1 High School, Handan 056001, Hebei Province, P. R. China
    3 Affiliated Secondary School of Shandong Normal University, Ji'nan 250014, P. R. China
  • Received:2018-07-26 Accepted:2018-08-19 Published:2019-03-09
  • Contact: Qikun ZHANG E-mail:zhangqk@sdnu.edu.cn
  • Supported by:
    山东师范大学本科生科研基金项目(2017BKSKY49);山东师范大学本科生科研基金项目(2018BKSKY54);大学生创新训练计划项目(20181501);山东师范大学教学改革项目:工程教育认证背景下化学工程与工艺专业建设与教学改革新探索

摘要:

氨氮含量的高低是衡量湖泊和水库水体富营养化的指标之一,水体中的氮元素极易诱发水体富营养化。目前,国标中通过比色法来检测氨氮含量的方法主要有靛酚蓝分光光度法和纳氏试剂分光光度法。根据高等教育国家标准中对于培养大学生的创新实践、科研意识、实验动手能力的要求,依照检测水体中氨氮含量的国家标准,检测了济南市区内三大名泉(趵突泉、黑虎泉、五龙潭)、大明湖、聊城东昌湖以及天津海河的水体样品中的氨氮浓度,并把山东师范大学的生活自来水作为对比样品,比较了比色法检测水体中氨氮含量的两种检测方法的差异。更重要的是,学生从检测过程中的得失领悟到了采用比色法检测、分析样品的方法技巧。结果表明,通过不断地完善检测方案,两种方法的标准曲线相关系数均达到0.9999以上。在实验操作中,由于操作过程和操作手法的不同(例如显色剂浓度、标准溶液的取用技巧以及各溶剂混合之后放置时间的长短等),获得了不同的实验结果,对实验结果的异常现象出现的原因进行了分析,同时讨论了两种检测方法的不同。

关键词: 靛酚蓝法, 纳氏试剂法, 比色法, 氨氮含量, 吸光度

Abstract:

The level of ammonia nitrogen source is one indicator of eutrophication of lakes and reservoirs. Currently, indigo phenol blue spectrophotometry and Nash reagent spectrophotometry have been adopted to detect the NH3-N content in national standard of the People's Republic of China. To cultivate college students' innovative practice, scientific research consciousness and experimental hands-on ability, the ammonia nitrogen concentration in the water samples of three famous springs (Baotu Spring, Heihu spring, Wulong pool), Daming Lake, Dongchang Lake and Haihe river was tested. Taking the drinking water from Shandong Normal University for comparison, the differences between the two methods were studied. More importantly, the students learned from the success and failure in the testing process that they used the colorimetric method to detect and analyze samples. The correlation coefficients of the standard curves of the two methods reached up to 0.9999 through continuous improvement of the detection scheme. In the experimental operation, different experimental results have been obtained due to the variation of operation and manipulation (such as the concentration of chromogenic agent, the operation skill of the standard solution and the length of the time after the mixing of each solvent etc.). This paper analyzes the causes of the outlier in the experimental results, and discusses the difference of two detection methods.

Key words: Indophenol blue spectrophotometry, Nessler's reagent method, Colorimetric method, Ammonia nitrogen concentration, Absorbance

MSC2000: 

  • G64