大学化学 >> 2019, Vol. 34 >> Issue (7): 67-72.doi: 10.3866/PKU.DXHX201812009

化学实验 上一篇    下一篇

碳点的制备及荧光量子产率的测定——一个仪器分析综合实验

陈芳*(),朱丽华,王宏   

  • 收稿日期:2018-12-12 发布日期:2019-02-20
  • 通讯作者: 陈芳 E-mail:fchen@hust.edu.cn
  • 基金资助:
    华中科技大学教学研究项目(17074);华中科技大学化学与化工学院教学团队培育项目(201601)

Preparation of Carbon Dots and Determination of Their Fluorescence Quantum Yield

Fang CHEN*(),Lihua ZHU,Hong WANG   

  • Received:2018-12-12 Published:2019-02-20
  • Contact: Fang CHEN E-mail:fchen@hust.edu.cn
  • Supported by:
    华中科技大学教学研究项目(17074);华中科技大学化学与化工学院教学团队培育项目(201601)

摘要:

碳点作为碳家族的新兴纳米材料,不仅具有独特的光学性质,而且具有易制备、原材料低廉、耐光漂白和生物相容性好等优良化学性质。本实验分别采用微波、水热和热解三种方法制备碳点,并采用红外光谱进行表征;分别测定三种碳点的紫外-可见吸收光谱和荧光光谱,并与有机荧光小分子进行比较;最后以硫酸奎宁为基准,测定三种碳点的荧光量子产率。该实验不仅让学生学习了碳纳米材料的制备方法、了解碳纳米材料独特的发光性能,还通过量子产率的测定使学生对三种分子光谱仪器的结构、原理和应用有更深入的理解。本实验可作为10-12学时的仪器分析综合实验,对化学、应用化学及材料化学等专业的大三学生开设。

关键词: 综合实验, 碳点, 荧光, 量子产率

Abstract:

As a new type of nano-materials, carbon dots have unique optical properties and excellent chemical properties, such as easy preparation, low cost, high chemical stability and good biocompatibility. Three kinds of carbon dots were prepared by microwave, hydrothermal and pyrolysis methods, and characterized by infrared spectroscopy. The ultraviolet-visible absorption spectra and fluorescence spectra of the three carbon dots were measured and compared with those of small organic fluorescent molecules. The fluorescence quantum yield of three carbon dots was measured using quinine sulfate as standards. This experiment not only enables students to learn the preparation methods and the unique luminescent properties of carbon nanomaterials, but also enables students to have a deeper understanding of the structures, principles and applications of three kinds of molecular spectroscopy instruments based on the measurement of quantum yield. This experiment can be used as a comprehensive 10-hour instrumental analysis experiment for junior students majoring in chemistry, applied chemistry and material chemistry.

Key words: Comprehensive experiment, Carbon dots, Fluorescence, Quantum yield

MSC2000: 

  • G64