大学化学 >> 2020, Vol. 35 >> Issue (4): 119-124.doi: 10.3866/PKU.DXHX201911062

所属专题: 全国大学生化学实验创新设计竞赛

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脂肪酶Novozym 435催化合成单月桂酸甘油酯

严子君, 张鑫, 吴祚骜, 吴凯群(), 王玉良   

  • 收稿日期:2019-11-27 录用日期:2019-12-16 发布日期:2020-02-12
  • 通讯作者: 吴凯群 E-mail:877279887@qq.com

Enzymatic Synthesis of Glycerol Monolaurate Catalyzed by Lipase Novozym 435

Zijun Yan, Xin Zhang, Zuoao Wu, Kaiqun Wu(), Yuliang Wang   

  • Received:2019-11-27 Accepted:2019-12-16 Published:2020-02-12
  • Contact: Kaiqun Wu E-mail:877279887@qq.com

摘要:

生物酶催化的有机化学反应具有选择性高、反应条件温和、环境友好等优点,本项目通过对酶催化合成单月桂酸甘油酯的反应条件和产物分离条件进行详细研究,将酶催化反应引入本科实验教学,使学生学习先进的知识技术。本项目的合成部分采用月桂酸:甘油=1:3.5(摩尔比)的投料比、5%酶用量,在52℃反应80 min;回收脂肪酶后,洗涤除去甘油,蒸馏除去叔丁醇,最后用石油醚重结晶可得到纯度90%以上的高含量产品,分离产率47%–53%,实验的稳定性和重现性好,很适合作为一个大学本科有机实验项目。

关键词: 生物酶, Novozym 435, 单月桂酸甘油酯

Abstract:

The potential of biocatalysis has been recognized as an efficient and green tool for modern organic synthesis due to its high selectivity, mild reaction conditions and sustainability. The enzyme-catalyzed synthesis of glycerol monolaurate were investigated in this study. The optimized reaction takes place with a 1:3.5 molar ratio of lauric acid to glycerol in the presence of 5% enzyme catalyst at 52℃ for 80 min. The product with 90% purity was obtained in 47%-53% yield via several separation steps. This enzymatic synthesis shows high stability and good repeatability, and is a suitable organic chemistry experiment for undergraduate laboratory teaching.

Key words: Lipase, Novozym 435, Glycerol monolaurate

MSC2000: 

  • G64