大学化学 >> 2021, Vol. 36 >> Issue (6): 2006075.doi: 10.3866/PKU.DXHX202006075

师生笔谈 上一篇    下一篇

刍议质谱技术发现新型蛋白质翻译后修饰研究在化学与生物学教学中的应用

王志鹏1,2,3,*(), 张璇4, 车子良5, 蒋振雄6, 马新雨2   

  1. 1 Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    2 Department of Chemistry, Texas A & M University, College Station, TX 77840, USA
    3 清华大学化学系, 北京 100084
    4 清华大学生命学院, 北京 100084
    5 Department of Mathematics, Harvard University, MA 02138, USA
    6 Department of Biology, Texas A & M University, College Station, TX 77840, USA
  • 收稿日期:2020-06-29 录用日期:2020-08-13 发布日期:2020-09-14
  • 通讯作者: 王志鹏 E-mail:wzpchem1991@gmail.com
  • 作者简介:王志鹏, Email: wzpchem1991@gmail.com

Discussion on the Application of Novel Protein Post-Translational Modification Discovered by Mass Spectrometry in Chemistry and Biology Education

Zhipeng A. Wang1,2,3,*(), Xuan Zhang4, Ziliang Che5, Zhenxiong Jiang6, Xinyu Ma2   

  1. 1 Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    2 Department of Chemistry, Texas A & M University, College Station, TX 77840, USA
    3 Department of Chemistry, Tsinghua University, Beijing 100084, China
    4 School of Life Sciences, Tsinghua University, Beijing 100084, China
    5 Department of Mathematics, Harvard University, MA 02138, USA
    6 Department of Biology, Texas A & M University, College Station, TX 77840, USA
  • Received:2020-06-29 Accepted:2020-08-13 Published:2020-09-14
  • Contact: Zhipeng A. Wang E-mail:wzpchem1991@gmail.com

摘要:

随着质谱技术的不断发展,质谱技术已经被广泛应用于化学、材料、生命科学等众多领域,特别是其在生物化学与化学生物学领域的应用极大扩展了研究人员对于蛋白质翻译后修饰的认知。本文从基本的质谱原理入手,着重探讨了质谱技术在以组蛋白为模式蛋白的赖氨酸翻译后修饰的发现过程,并展示如何将基础有机化学和生物化学知识融入前沿科学研究。本文通过梳理不同的研究逻辑下质谱技术在蛋白翻译后修饰的研究中发挥的作用,旨在引导学生建立起将基础教学内容与实际应用相联系的意识及思维模式。

关键词: 质谱技术, 串联质谱, 翻译后修饰, 生物化学

Abstract:

Rapid development of mass spectrum technology (MS) brings about its wide applications in many fields such as chemistry, material science and life sciences, and its application in biochemistry and chemical biology have been greatly expanding the knowledge of novel protein post-translation modification (PTM). This manuscript starts from the basic principles of mass spectrum and later elaborates the application of MS in discovering lysine post-translation modifications over histones as model proteins, showing a path incorporating fundamental organic chemistry and biochemistry knowledge into cutting-edge researches. By analyzing roles of mass spectrum throughout post-translation modification studies under different research logistics, this manuscript was aimed to help establishing a way of thinking that associates fundamental chemistry and biology education with practical applications for students.

Key words: Mass spectrum technology, MS/MS, PTM, Biochemistry

MSC2000: 

  • G64