大学化学 >> 2021, Vol. 36 >> Issue (6): 2006087.doi: 10.3866/PKU.DXHX202006087

化学实验 上一篇    下一篇

蓝藻FBP/SBPase的制备及其与配体相互作用的分析——科研转化的化学生物学综合实验设计

冯玲玲(), 陈琼, 李玉梦, 梁景晗, 王舟   

  • 收稿日期:2020-06-29 录用日期:2020-08-02 发布日期:2020-09-03
  • 通讯作者: 冯玲玲 E-mail:fll708@mail.ccnu.edu.cn
  • 作者简介:冯玲玲, Email: fll708@mail.ccnu.edu.cn
  • 基金资助:
    国家自然科学基金面上项目(21877046);国家自然科学基金面上项目(21072073);中央高校基本科研业务费专项资金资助科研转化为教学项目(CCNU16KYZHSY22);教学研究项目(201619)

Preparation of an Important Enzyme FBP/SBPase from Cyanobacteria and Analysis of Interaction between Target Enzyme and Ligand: One Comprehensive Experiment Based on Research

Lingling Feng(), Qiong Chen, Yumeng Li, Jinghan Liang, Zhou Wang   

  • Received:2020-06-29 Accepted:2020-08-02 Published:2020-09-03
  • Contact: Lingling Feng E-mail:fll708@mail.ccnu.edu.cn

摘要:

以蓝藻果糖-1,6-/景天庚酮糖-1,7-二磷酸酶为靶酶,异源表达获得靶酶,分析靶酶的纯度及含量,探讨配体与靶酶的相互作用。通过本实验,学生掌握获取靶酶的方法,掌握靶酶与配体相互作用的分析方法,了解药物筛选模型建立的研究思路及实验技术,提升分析、解决问题及综合运用知识的能力,增强科研服务社会意识及责任感。

关键词: 化学生物学, 综合实验设计, 靶酶与配体相互作用, 果糖-1, 6-/景天庚酮糖-1, 7-二磷酸酶

Abstract:

Cyanobacteria fructose-1, 6-/sedoheptanose-1, 7-diphosphatase (Cy-FBP/SBPase) was used as the target enzyme, which was obtained through heterologous expression and purification. The purity and content of the target enzyme and the interaction between the enzyme and its ligands (substrate, metal ion and inhibitor) were analyzed. Through the experimental study and exploration, students can master the biosynthesis method to obtain the target enzyme, master the method to analyze the interaction between the target enzyme and its ligand, understand research ideas and experimental techniques needed to establish a drug screening model based on the target enzyme, upgrade the abilities to analyze and solve problems and use chemical biology knowledge, strengthen the social consciousness of scientific research service and social responsibility.

Key words: Chemical biology, Comprehensive experimental design, Interaction between target enzyme and ligand, Fructose-1, 6-/sedoheptanose-1, 7-diphosphatase

MSC2000: 

  • G64