大学化学 >> 2021, Vol. 36 >> Issue (9): 2011055.doi: 10.3866/PKU.DXHX202011055

所属专题: 分析化学课程与实验教学

化学实验 上一篇    下一篇

超高效合相色谱法测定咖啡因含量实验设计

谢一凡1, 姚莉韵1,2,*(), 严忠红2, 蔡玉兴1, 金玉杰1, 李宁1   

  1. 1 上海交通大学医学院, 基础医学实验教学中心, 上海 200025
    2 上海交通大学医学院, 药物化学与生物信息学中心, 上海 200025
  • 收稿日期:2020-11-25 录用日期:2021-12-28 发布日期:2021-01-15
  • 通讯作者: 姚莉韵 E-mail:yaoliyun517@163.com
  • 作者简介:姚莉韵, Email: yaoliyun517@163.com
  • 基金资助:
    上海交通大学医学院课程建设基金项目(BJ13000180095)

Design of Experiment for Determination of Caffeine Content by Ultra Performance Convergence Chromatography

Yifan Xie1, Liyun Yao1,2,*(), Zhonghong Yan2, Yuxing Cai1, Yujie Jin1, Ning Li1   

  1. 1 Basic Medical Experimental Teaching Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
    2 Medicinal Chemistry and Bioinformatics Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
  • Received:2020-11-25 Accepted:2021-12-28 Published:2021-01-15
  • Contact: Liyun Yao E-mail:yaoliyun517@163.com

摘要:

设计了一个仪器分析实验,利用超高效合相色谱仪(Ultra Performance Convergence Chromatography,UPCC或UPC2)测定饮料中咖啡因的含量。该实验选取可乐、绿茶、乌龙茶三种饮料,样品用甲醇萃取,萃取液无需旋蒸,直接稀释定容后进样,测定其中咖啡因的含量。通过该实验学生不仅能巩固称量、定容、移液等基本操作,还能掌握UPC2的基本原理、操作及应用色谱软件处理数据的技能。本实验将新型色谱仪UPC2应用于基础实验教学,可以拓展学生的科学视野,有利于培养学生的创新精神和实践能力。

关键词: 超高效合相色谱, 咖啡因, 含量测定, 实验教学

Abstract:

An experiment of instrumental analysis for determination of caffeine content in beverages by Ultra Performance Convergence Chromatography (UPC2) was designed. The caffeine in three beverages including coke, green tea and oolong tea was extracted and diluted by methanol. Then caffeine concentrations in beverages were measured by UPC2. Through this experiment, students could not only consolidate several laboratory skills such as weighing, constant volume and liquid transfer, but also master the basic theory, operation and data processing of UPC2. This experiment integrates the new chromatography into basic laboratory teaching, which can broaden students' scientific horizons. It is conductive to cultivating students' innovative spirit and practical ability.

Key words: UPC2, Caffeine, Content determination, Laboratory teaching

MSC2000: 

  • G64