大学化学 >> 2022, Vol. 37 >> Issue (5): 2110089.doi: 10.3866/PKU.DXHX202110089

所属专题: 第2届全国大学生化学实验创新设计竞赛

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鲁米诺的合成与振荡化学发光

张一平, 邓昆月, 赵博轩, 张雨豪, 陈洲慧, 阮永红, 周金梅(), 张慧君(), 林敏, 邓顺柳, 曹晓宇, 任艳平   

  • 收稿日期:2021-10-28 录用日期:2021-11-04 发布日期:2022-03-22
  • 通讯作者: 周金梅,张慧君 E-mail:jmzhou@xmu.edu.cn;meghjzhang@xmu.edu.cn
  • 作者简介:Email: meghjzhang@xmu.edu.cn (张慧君)
    Email: jmzhou@xmu.edu.cn (周金梅)
  • 基金资助:
    2021年度教育部“基础学科拔尖学生培养计划2.0”重点课题(20211038);2021年厦门大学教学改革研究项目(JG20210127);2020年厦门大学一流本科课程项目

Synthesis of Luminol and Chemiluminescence Oscillation

Yiping Zhang, Kunyue Deng, Boxuan Zhao, Yuhao Zhang, Zhouhui Chen, Yonghong Ruan, Jinmei Zhou(), Huijun Zhang(), Min Lin, Shunliu Deng, Xiaoyu Cao, Yanping Ren   

  • Received:2021-10-28 Accepted:2021-11-04 Published:2022-03-22
  • Contact: Jinmei Zhou,Huijun Zhang E-mail:jmzhou@xmu.edu.cn;meghjzhang@xmu.edu.cn

摘要:

鲁米诺的合成与化学发光是一个极具展示度的基础化学实验。近年来,国内部分高校陆续开设了这一实验,引起不错的反响。为进一步丰富和提升该实验的科学理论内涵,我们进行了如下改进:1) 在合成部分引入机械搅拌、硫粉还原和微波合成,实现安全、快速、高效制备高纯度鲁米诺的目的;2) 在单次化学发光的基础上,特别引入宛如星辰闪烁般的振荡化学发光反应(鲁米诺/H2O2/SCN/Cu2+/OH振荡体系),该反应涉及非平衡态热力学和非线性化学等重要概念与基础理论知识,激发学生的求知欲;3) 设计并搭建了可与荧光光谱仪配套使用的简易微型磁力搅拌加热装置,实现了对振荡化学发光的光强和振荡周期等数据的实时定量监测,使学生可以自行设计对比实验,探究影响振荡反应过程的因素,并总结振荡反应规律。该实验涵盖了多学科的重要知识点,兼具科学性与趣味性,对培养学生的探索精神和创新意识具有重要意义。

关键词: 硫粉还原, 微波合成, 振荡化学发光, 光强和周期实时监测

Abstract:

Synthesis and investigation of the chemiluminescence of Luminol is a valuable educational and pedagogical tool. In recent years, a few domestic universities started offering this experiment to undergraduate students. To further enrich the scientific and theoretical understanding of the synthetic and chemiluminescent process, we have improved the existing experiments in several ways. First, we introduced mechanical stirring, sulfur-mediated reduction, and microwave chemistry in the synthesis portion to achieve safe, rapid, and efficient preparation of high purity Luminol. Second, we introduced the Luminol/H2O2/SCN/Cu2+/OH system for chemiluminescence oscillation, which involves applying important concepts such as kinetics, autocatalysis, non-equilibrium thermodynamics, and dissipative structures. Finally, to achieve real-time quantitative monitoring of the light intensity and frequency of the chemiluminescence oscillating process, we designed a simple miniature magnetic stirring heating device, which can be used in conjunction with a fluorescence spectrometer. Students can design comparison experiments independently and explore the mechanism. This experiment involves combining several important multidisciplinary chemical concepts. The precise measurement of the chemiluminescence oscillating process has effectively captured the interest of students.

Key words: Sulfur-mediated reduction, Microwave-assisted synthesis, Chemiluminescence oscillation, Real-time monitor

MSC2000: 

  • G64