大学化学 >> 2022, Vol. 37 >> Issue (7): 2111089.doi: 10.3866/PKU.DXHX202111089

化学实验 上一篇    下一篇

生物纳米孔道单分子多肽磷酸化识别与测量的创新综合实验

胡正利1,3, 辛凯莉1, 刘少创1, 钟诚兵1, 武雪原1, 应佚伦1,2, 孔璇凤1, 余晓冬1, 张剑荣1, 龙亿涛1,*()   

  1. 1 南京大学化学化工学院, 生命分析化学国家重点实验室, 南京 210023
    2 南京大学化学和生物医药创新研究院, 南京 210023
    3 南京大学深圳研究院, 广东 深圳 518057
  • 收稿日期:2021-11-30 录用日期:2021-12-13 发布日期:2022-02-14
  • 通讯作者: 龙亿涛 E-mail:yitaolong@nju.edu.cn
  • 作者简介:龙亿涛, Email: yitaolong@nju.edu.cn
  • 基金资助:
    国家自然科学基金(22027806);国家自然科学基金(21834001);广东省基础与应用基础研究基金(2020A1515110298)

A Comprehensive Single-molecule Experiment of Peptide Phosphorylation Detection with Biological Nanopores

Zhengli Hu1,3, Kaili Xin1, Shaochuang Liu1, Chengbing Zhong1, Xueyuan Wu1, Yi-Lun Ying1,2, Xuanfeng Kong1, Xiaodong Yu1, Jianrong Zhang1, Yi-Tao Long1,*()   

  1. 1 State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China
    2 Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing 210023, China
    3 Shenzhen Research Institute of Nanjing University, Shenzhen 518057, Guangdong Province, China
  • Received:2021-11-30 Accepted:2021-12-13 Published:2022-02-14
  • Contact: Yi-Tao Long E-mail:yitaolong@nju.edu.cn

摘要:

生物纳米孔道技术是一种具有革命性意义的单分子电化学分析方法,经过近三十年的发展,已经能够实现单分子DNA测序并广泛应用于多肽、蛋白质等单个生物分子的检测研究。本文探讨了将纳米孔道单分子测量方法引入大学实验教学的必要性与重要性,介绍了生物纳米孔道单分子实验的教学意义、目的、内容及其组织实施方式。本实验采用“科教融合”的教学模式,针对具有一定化学、生物化学基础知识并对科研感兴趣的二、三年级本科生,精心设计并不断优化教学方案,将纳米孔道单分子电化学及相关交叉领域的最新研究成果融入课堂教学,拓展学生的科研视野、激发学生的科研兴趣。此外,通过教师引导,鼓励学生大胆提出问题,自主探索,合作完成实验内容,培养学生的学术思维和综合创新能力。

关键词: 生物纳米孔道, 单分子检测, 多肽磷酸化, 科教融合, 化学综合实验

Abstract:

The biological nanopore technique is a revolutionary single-molecule electrochemical method. The single-molecule sequencing of DNA was accomplished following rapid developments over the last three decades, and has been extensively applied in the single-molecule sensing of biomolecules, including peptides and proteins. This paper discusses the importance of introducing nanopore-based single-molecule experiments into university classrooms and expounds on the specific content and implementation model for teaching. This innovative experimental course adopts the teaching mode of "integration of science and education" for second- and third-year undergraduates with some background in chemistry and biochemistry. The teaching plan has been carefully designed and is continuously being optimized according to teaching practices. We integrated the latest achievements in nanopore electrochemistry and related interdisciplinary fields into classroom teaching to expand students' horizons and stimulate their interests in scientific research. In addition, students were encouraged to collaborate on experimental assignments and brainstorm, which could help cultivate their academic thinking capability and comprehensive innovation ability.

Key words: Biological nanopore, Single-molecule measurement, Peptide phosphorylation, Integration of science and education, Comprehensive chemistry experiment

MSC2000: 

  • G64