大学化学 >> 2022, Vol. 37 >> Issue (9): 2204087.doi: 10.3866/PKU.DXHX202204087

所属专题: 化学科普(2022)

科普 上一篇    下一篇

DNA历险记——氧化损伤篇

关喆, 宋红杰, 刘睿()   

  • 收稿日期:2022-04-24 录用日期:2022-06-04 发布日期:2022-09-02
  • 通讯作者: 刘睿 E-mail:liur@scu.edu.cn
  • 作者简介:刘睿, Email: liur@scu.edu.cn
  • 基金资助:
    四川大学大学生创新训练计划项目(20220311L);四川大学研究生教育教学改革研究项目(GSSCU2021097);四川大学化学学院2021年度教育教学改革研究项目(202101)

Adventures of DNA: Oxidative Damage

Zhe Guan, Hongjie Song, Rui Liu()   

  • Received:2022-04-24 Accepted:2022-06-04 Published:2022-09-02
  • Contact: Rui Liu E-mail:liur@scu.edu.cn

摘要:

DNA损伤指生物体生命过程中由外源因素或内源因素导致的DNA结构改变,包括DNA烷基化、氧化、错配、断裂等。虽然生物体内存在着DNA损伤修复机制,能在一定程度上修复损伤的DNA,但假如未被修复,积累起来的损伤将严重威胁生物体基因组的稳定性和生命安全。DNA氧化性损伤是最常见的损伤之一,而8-羟基脱氧鸟苷可以看作DNA氧化损伤的生物标志物,对8-羟基脱氧鸟苷的定量检测能够反映DNA氧化损伤的程度,对疾病的早期诊断和治疗至关重要。本文结合情景故事介绍了8-羟基脱氧鸟苷的产生过程、修复机制和检测手段。

关键词: DNA损伤与修复, DNA氧化性损伤, 8-羟基脱氧鸟苷, 产生过程, 修复机制, 检测手段

Abstract:

DNA damage is a change in DNA structure caused by external or internal factors, including DNA alkylation, oxidation, mismatch, breakage. Although the DNA damage repair mechanism can repair damaged DNA to a certain extent, accumulated damage that is not repaired can seriously threaten genome stability and human life. Oxidative DNA damage is one of the most common types of damage, with 8-hydroxydeoxyguanosine as its biomarker. The quantitative detection of 8-hydroxydeoxyguanosine can reflect the degree of oxidative DNA damage, which is very important for the early diagnosis and treatment of diseases. In this paper, we introduce the production process, repair mechanism, and detection methods for 8-hydroxydeoxyguanosine.

Key words: DNA damage and repair, Oxidative DNA damage, 8-hydroxydeoxyguanosine, Production process, Repair mechanism, Detection methods

MSC2000: 

  • G64