大学化学 >> 2015, Vol. 30 >> Issue (5): 39-44.doi: 10.3866/pku.DXHX20150539

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MALDI-TOF MS测定多肽类聚合物相对分子质量的条件优化

余惠娟1, 褚曰环1, 邵光1, 江帆2, 巢晖1   

  1. 1. 中山大学化学与化学工程学院 广东广州 510275;
    2. 深圳市出入境检验检疫局工业品检测技术中心 广东深圳 518017
  • 发布日期:2015-10-24

Optimization for Determination of PolypeptidesRelative Molecular Mass by MALDI-TOF MS

Yu Huijuan1, Chu Yuehuan1, Shao Guang1, Jiang Fan2, Chao Hui1   

  1. 1. School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, Guangdong, China;
    2. The Testing and Technology Center for Industrial Products, Shenzhen Enter-exit Inspection and Quarantine Bureau, Shenzhen 518017, Guangdong, China
  • Published:2015-10-24

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摘要：

本文用基质辅助激光解吸电离飞行时间质谱来测定多肽类聚合物的相对分子质量, 对基质、溶剂以及添加阳离子条件进行了优化。

关键词: 基质辅助激光解吸电离飞行时间质谱, 多肽类聚合物, 相对分子质量测定

Abstract:

In this paper, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI- TOF MS) was used for measuring the relative molecular mass of polypeptides, and the matrixes, solvents, and adding cationic reagents were investigated for optimized determinations.

Key words: Matrix-assisted laser desorption/ionization time of flight mass spectrometry, Polypeptides, Deter- mination of relative molecular mass

MSC2000: 

  • O6