大学化学 >> 2018, Vol. 33 >> Issue (1): 1-6.doi: 10.3866/PKU.DXHX201711035

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冷冻电镜技术应用于生物分子高分辨结构解析——2017年诺贝尔化学奖浅谈

李治非,高宁*()   

  • 收稿日期:2017-11-28 发布日期:2018-01-13
  • 通讯作者: 高宁 E-mail:gaon@pku.edu.cn

Cryo-EM Method for High-Resolution Structure Determination: A Brief Introduction to 2017 Nobel Prize in Chemistry

Zhifei LI,Ning GAO*()   

  • Received:2017-11-28 Published:2018-01-13
  • Contact: Ning GAO E-mail:gaon@pku.edu.cn

摘要:

2017年的诺贝尔化学奖颁发给了瑞士洛桑大学的Jacques Dubochet、美国哥伦比亚大学的Joachim Frank和英国医学研究理事会分子生物学实验室的Richard Henderson,以表彰他们在发展冷冻电镜技术用来解析处于天然状态的生物分子的高分辨率结构方面的贡献。冷冻电镜技术是在超低温下将含有生物分子样品的薄水膜迅速冷冻成玻璃态的薄冰膜,使得生物分子样品刚好包埋在薄冰膜中,再利用电子显微镜照射生物分子样品,在探测器上采集到生物样品的二维投影或衍射花样图片,最后通过三维重构的算法将二维投影或衍射花样的图片处理得到生物分子的三维结构的技术。

关键词: 冷冻电镜, 生物大分子, 结构生物学, 诺贝尔化学奖

Abstract:

The 2017 Nobel Prize in chemistry was awarded to Jacques Dubochet, Joachim Frank and Richard Henderson for their contribution to developing the cryo-electron microscopy (cryo-EM) method for high-resolution structure determination of biomolecules in solution. In recent years, cryo-EM is leading a revolution in structural biology, and becoming a major tool in studying the structure and function of biomolecular machines. Here, we briefly describe the method development of cryo-EM and the personal contributions of the three Nobel Laureates.

Key words: Cryo-electron microscopy, Biomolecules, Structural biology, Nobel Prize in chemistry

MSC2000: 

  • G64