大学化学 >> 2019, Vol. 34 >> Issue (1): 1-6.doi: 10.3866/PKU.DXHX201811008

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浅谈2018年诺贝尔化学奖:驾驭进化的力量

周颖,朱理源,邹鹏*()   

  • 收稿日期:2018-11-12 发布日期:2019-01-16
  • 通讯作者: 邹鹏 E-mail:zoupeng@pku.edu.cn
  • 基金资助:
    基金委重大研究计划-培育项目(91753131);国家青年千人计划

A Brief Introduction to Nobel Prize in Chemistry 2018: Harnessing the Power of Evolution

Ying ZHOU,Liyuan ZHU,Peng ZOU*()   

  • Received:2018-11-12 Published:2019-01-16
  • Contact: Peng ZOU E-mail:zoupeng@pku.edu.cn
  • Supported by:
    基金委重大研究计划-培育项目(91753131);国家青年千人计划

摘要:

定向分子进化是蛋白质工程领域的一项关键技术。通过在试管中模拟并加速自然界中的生物进化过程,定向进化技术直接针对生物大分子的功能进行突变改造和筛选,从中发现具有特殊功能的多肽和蛋白质。2018年的诺贝尔化学奖颁发给了美国加州理工学院的弗朗西斯·阿诺德、美国密苏里大学的乔治·史密斯和英国医学研究理事会分子生物学实验室的格里高利·温特,以表彰他们在定向分子进化领域做出的开创性贡献。本文简述了定向分子进化技术的发展历程及其对绿色能源和生物医药行业的重要贡献。

关键词: 定向进化, 酶, 抗体, 噬菌体展示, 诺贝尔化学奖

Abstract:

In living organisms, protein functions are constantly evolving over generations throughout the history. Through iterative rounds of genetic mutations and natural selection of fit phenotypes, protein functions have been gradually optimized. This process could be mimicked and even greatly accelerated in the laboratory, when the selection pressure is directly applied to biomolecules of interest, which forms the basis of a technique called directed evolution. The Nobel Prize in chemistry 2018 was awarded jointly to Frances Arnold, George Smith and Gregory P. Winter for their pioneering contributions to the development and applications of directed evolution. Here we briefly review the history of this technique and its impact on renewable energy and pharmaceutical industry.

Key words: Directed evolution, Enzyme, Antibody, Phage display, Nobel Prize in chemistry

MSC2000: 

  • G64