大学化学 >> 2021, Vol. 36 >> Issue (8): 2011032.doi: 10.3866/PKU.DXHX202011032

化学实验 上一篇    下一篇

基于半导体聚合物纳米粒子探针的比率型活性物种检测——推荐一个研究型综合化学实验

宋国胜(), 廖师夷, 王友娟, 宦双燕()   

  • 收稿日期:2020-11-09 录用日期:2020-12-23 发布日期:2021-01-07
  • 通讯作者: 宋国胜,宦双燕 E-mail:songgs@hnu.edu.cn;syhuan@hnu.edu.cn
  • 作者简介:Email: syhuan@hnu.edu.cn (宦双燕)
    Email: songgs@hnu.edu.cn (宋国胜)
  • 基金资助:
    国家自然科学基金(21804039);2020年湖南省普通高等学校课程思政建设研究项目(HNKCSZ-2020-0074)

Semiconducting Polymer Nanoprobe for Ratiometric Detection of Reactive Oxygen Species: A Comprehensive Experiment

Guosheng Song(), Shiyi Liao, Youjuan Wang, Shuangyan Huan()   

  • Received:2020-11-09 Accepted:2020-12-23 Published:2021-01-07
  • Contact: Guosheng Song,Shuangyan Huan E-mail:songgs@hnu.edu.cn;syhuan@hnu.edu.cn

摘要:

介绍一个从纳米探针的合成、表征、性质测试到应用研究的完整研究型综合实验。本实验利用纳米共沉淀法制备了有机聚合物纳米探针,用有机半导体聚合物(PFODBT)作为参比分子和荧光染料分子(IR775s)作为响应分子,构建吸光度和荧光双比率型探针,实现了对活性氧物种(ClO-和ONOO-)含量的检测。采用紫外-可见吸收光谱仪可实现比率型吸光度检测活性氧物种的含量。利用PFODBT和IR775s之间的荧光共振能量转移(FRET)原理,采用荧光光谱仪可实现比率型荧光检测活性氧物种的含量。该实验综合性强、操作简便、可行性好、有利于培养学生全面的实验技能和科研能力。

关键词: 分析检测, 纳米探针, 荧光共振能量转移, 比率型检测, 活性物种

Abstract:

This works introduces a comprehensive research experiment about the synthesis and characterization of nanoprobes for detecting reactive oxygen species (ROS). In this experiment, organic semiconducting polymer nanoprobes were prepared using nano-coprecipitation method. Those nanoprobes can be employed for ratiometric detection of ROS, using semiconductor polymer (PFODBT) as the reference and fluorescent molecules (IR775s) as responsive unit. Specifically, the content of ROS (ClO- and ONOO-) can be measured by calculating PFODBT-to-IR775s absorbance ratio. Moreover, based on the principle of fluorescence resonance energy transfer (FRET) between PFODBT and IR775s, the ratio of fluorescent emission of PFODBT/IR775s can also be used for fluorescence ratiometric detection of ROS. This experiment is comprehensive and feasible, and benefited for training the solid experimental skills and scientific research ability for students.

Key words: Analytical measurement, Nanoprobe, Fluorescence resonance energy transfer, Ratiometric detection, Reactive oxygen species

MSC2000: 

  • G64