大学化学 >> 2021, Vol. 36 >> Issue (9): 2101012.doi: 10.3866/PKU.DXHX202101012

所属专题: 分析化学课程与实验教学

化学实验 上一篇    下一篇

紫外-可见分光光度法测定含铜废水中的铜离子

章子恒, 胡然, 马艺瑾, 孙兴蓉, 马思维, 朱忍, 曹秋娥, 周川华()   

  • 收稿日期:2021-01-07 录用日期:2021-01-27 发布日期:2021-02-22
  • 通讯作者: 周川华 E-mail:chzhou@ynu.edu.cn
  • 作者简介:周川华, Email: chzhou@ynu.edu.cn

An Experiment for Determining Copper(Ⅱ) Ions in Copper-Containing Sewage by UV-Vis Spectrophotometry

Ziheng Zhang, Ran Hu, Yijin Ma, Xingrong Sun, Siwei Ma, Ren Zhu, Qiue Cao, Chuanhua Zhou()   

  • Received:2021-01-07 Accepted:2021-01-27 Published:2021-02-22
  • Contact: Chuanhua Zhou E-mail:chzhou@ynu.edu.cn

摘要:

设计了一个通过紫外-可见分光光度法检测含铜废水中铜离子含量的本科教学实验。该实验基于芬顿反应原理,检测液中铜离子的存在可催化过氧化氢分解产生羟基自由基,后者将溶液中无色的3,3’,5,5’-四甲基联苯胺(TMB)氧化为黄色的TMB2+,利用TMB2+在452 nm处的吸光度,即可实现对含铜废水中铜离子的定量分析。本实验操作简便、测定时间短、稳定性较好且安全环保,符合本科教学实验的基本要求。

关键词: 芬顿反应, 铜离子检测, 紫外-可见分光光度法, 本科教学实验

Abstract:

A teaching experiment for determining copper(Ⅱ) ions in copper-containing sewage by UV-Vis spectrophotometry is designed. The experiment is performed based on the Fenton reaction principle, where the presence of copper(Ⅱ) ions in the assay solution catalyzes the decomposition of hydrogen peroxide to produce hydroxyl radicals. The latter oxidizes the colorless 3, 3', 5, 5'-tetramethylbenzidine (TMB) in the solution to the yellow TMB2+, which can be used for quantitative analysis copper(Ⅱ) ions in copper-containing sewage by measuring the absorbance of TMB2+ at 452 nm. With easy operation, short measurement time, excellent stability, eco-friendliness, this experiment conforms to the basic requirements of undergraduate laboratory teaching.

Key words: Fenton reaction, Cu(Ⅱ) detection, Ultraviolet-visible spectrophotometry, Undergraduate teaching experiment

MSC2000: 

  • G64