大学化学 >> 2021, Vol. 36 >> Issue (9): 2107023.doi: 10.3866/PKU.DXHX202107023

所属专题: 分析化学课程与实验教学

化学实验 上一篇    下一篇

手性气相色谱法测定野菊花中的龙脑对映体——推荐一个仪器分析实验

李会香, 刘莎莎, 雷杰()   

  • 收稿日期:2021-06-30 录用日期:2021-08-17 发布日期:2021-08-26
  • 通讯作者: 雷杰 E-mail:jielei@fudan.edu.cn
  • 作者简介:雷杰, Email: jielei@fudan.edu.cn
  • 基金资助:
    教育部2019年第一批产学合作协同育人项目(201901102005);复旦大学2021年度第一批本科教学研究与改革实践项目(FD2021A115)

Determination of Borneol Isomer in Wild Chrysanthemum by Chiral Gas Chromatography: Recommending an Instrumental Analysis Experiment

Huixiang Li, Shasha Liu, Jie Lei()   

  • Received:2021-06-30 Accepted:2021-08-17 Published:2021-08-26
  • Contact: Jie Lei E-mail:jielei@fudan.edu.cn

摘要:

推荐了一个仪器分析实验“手性气相色谱法测定野菊花中的龙脑对映体”。实验采用手性毛细管气相色谱柱,对野菊花中的(+)-龙脑和(-)-龙脑进行检测并定量。实验内容包括:野菊花挥发油的提取、色谱程序升温条件的优化、内标标准曲线法的应用等。本实验从分析化学的角度,重点介绍了手性色谱柱的种类及分离原理,建议作为拓展类的仪器分析实验开设。

关键词: 手性色谱法, 野菊花, 龙脑对映体, 分析化学实验

Abstract:

An instrumental analysis experiment "Determination of borneol isomer in wild chrysanthemum by chiral capillary gas chromatography" is recommended. Determination and quantitative analysis of (+)-borneol and (-)-borneol in wild chrysanthemum were conducted using chiral capillary gas chromatographic column in this experiment. Experimental contents consisted of extraction of volatile oil of wild chrysanthemum, optimization of heating program, application of internal standard curve method, etc. From the perspective of analytical chemistry, this experiment focuses on the types and separation principle of chiral chromatographic columns, and it is suggested to be set up as an expanding instrumental analysis experiment.

Key words: Chiral chromatography, Wild chrysanthemum, Borneol isomer, Analytical chemistry experiment

MSC2000: 

  • G64