University Chemistry ›› 2017, Vol. 32 ›› Issue (8): 42-48.doi: 10.3866/PKU.DXHX201701021

• Chemistry Laboratory • Previous Articles     Next Articles

Characterization of DNA Polymerases Activities by DNA Genetic Analyzer

Yang LIU*()   

  • Published:2017-08-15
  • Contact: Yang LIU E-mail:yliu442@tsinghua.edu.cn
  • Supported by:
    清华大学2016秋本科教学改革项目;清华大学实验室创新基金;清华大学基础学科拔尖学生培养实验计划(学堂班)

Abstract:

In this paper, one comprehensive experiment was proposed for teaching the chemical biology laboratory. By utilizing 6-FAM labelled primer and DNA genetic analyzer, we studied the effects of DNA polymerases structure on the replication of damaged DNA. The application of this experiment to laboratory teaching can cultivate students' comprehensive analysis and hands-on abilities.

Key words: Benzopyrene, Translesion DNA synthesis, Teaching experiment

MSC2000: 

  • G64