Abstract:

In this program, a copper metal-organic framework material with fluorescence performance was synthesized and used to construct a multicolor fluorescent assay for alkaline phosphatase activity based on a smartphone. This assay was applied to the visual detection of alkaline phosphatase content in serum. Undergraduates have achieved the fullest training and knowledge during this program. This paper introduces in detail the topic selection, experimental scheme design, experimental process, achievements and experience gained by students in this project.

Key words: Research-training program, Alkaline phosphatase, Multicolor fluorescent biosensor, Visual detection, Mobile applications