脂肪酶Novozym 435催化合成单月桂酸甘油酯

doi:10.3866/PKU.DXHX201911062

Abstract

Abstract:

The potential of biocatalysis has been recognized as an efficient and green tool for modern organic synthesis due to its high selectivity, mild reaction conditions and sustainability. The enzyme-catalyzed synthesis of glycerol monolaurate were investigated in this study. The optimized reaction takes place with a 1:3.5 molar ratio of lauric acid to glycerol in the presence of 5% enzyme catalyst at 52℃ for 80 min. The product with 90% purity was obtained in 47%-53% yield via several separation steps. This enzymatic synthesis shows high stability and good repeatability, and is a suitable organic chemistry experiment for undergraduate laboratory teaching.

Key words: Lipase, Novozym 435, Glycerol monolaurate

MSC2000:

Zijun Yan, Xin Zhang, Zuoao Wu, Kaiqun Wu, Yuliang Wang. Enzymatic Synthesis of Glycerol Monolaurate Catalyzed by Lipase Novozym 435[J].University Chemistry, 2020, 35(4): 119-124.

Figures/Tables 3

References 22

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石油醚/mL	第一次洗涤	单酯含量/%	产率/%	第二次洗涤	单酯含量/%	产率/%
4	1.5 mL石油醚(冰)	87.17	54.20	1.5 mL石油醚(冰)	90.60	50.07
5	1.5 mL石油醚(冰)	90.67	52.88	1.5 mL石油醚(冰)	91.53	50.44

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Enzymatic Synthesis of Glycerol Monolaurate Catalyzed by Lipase Novozym 435

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