University Chemistry ›› 2020, Vol. 35 ›› Issue (4): 119-124.doi: 10.3866/PKU.DXHX201911062

Special Issue: 全国大学生化学实验创新设计竞赛

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Enzymatic Synthesis of Glycerol Monolaurate Catalyzed by Lipase Novozym 435

Zijun Yan, Xin Zhang, Zuoao Wu, Kaiqun Wu(), Yuliang Wang   

  • Received:2019-11-27 Accepted:2019-12-16 Published:2020-02-12
  • Contact: Kaiqun Wu E-mail:877279887@qq.com

Abstract:

The potential of biocatalysis has been recognized as an efficient and green tool for modern organic synthesis due to its high selectivity, mild reaction conditions and sustainability. The enzyme-catalyzed synthesis of glycerol monolaurate were investigated in this study. The optimized reaction takes place with a 1:3.5 molar ratio of lauric acid to glycerol in the presence of 5% enzyme catalyst at 52℃ for 80 min. The product with 90% purity was obtained in 47%-53% yield via several separation steps. This enzymatic synthesis shows high stability and good repeatability, and is a suitable organic chemistry experiment for undergraduate laboratory teaching.

Key words: Lipase, Novozym 435, Glycerol monolaurate

MSC2000: 

  • G64