University Chemistry ›› 2021, Vol. 36 ›› Issue (6): 2006087.doi: 10.3866/PKU.DXHX202006087

• Chemistry Laboratory • Previous Articles     Next Articles

Preparation of an Important Enzyme FBP/SBPase from Cyanobacteria and Analysis of Interaction between Target Enzyme and Ligand: One Comprehensive Experiment Based on Research

Lingling Feng(), Qiong Chen, Yumeng Li, Jinghan Liang, Zhou Wang   

  • Received:2020-06-29 Accepted:2020-08-02 Published:2020-09-03
  • Contact: Lingling Feng E-mail:fll708@mail.ccnu.edu.cn

Abstract:

Cyanobacteria fructose-1, 6-/sedoheptanose-1, 7-diphosphatase (Cy-FBP/SBPase) was used as the target enzyme, which was obtained through heterologous expression and purification. The purity and content of the target enzyme and the interaction between the enzyme and its ligands (substrate, metal ion and inhibitor) were analyzed. Through the experimental study and exploration, students can master the biosynthesis method to obtain the target enzyme, master the method to analyze the interaction between the target enzyme and its ligand, understand research ideas and experimental techniques needed to establish a drug screening model based on the target enzyme, upgrade the abilities to analyze and solve problems and use chemical biology knowledge, strengthen the social consciousness of scientific research service and social responsibility.

Key words: Chemical biology, Comprehensive experimental design, Interaction between target enzyme and ligand, Fructose-1, 6-/sedoheptanose-1, 7-diphosphatase

MSC2000: 

  • G64