基于半导体聚合物纳米粒子探针的比率型活性物种检测——推荐一个研究型综合化学实验

doi:10.3866/PKU.DXHX202011032

Abstract

Abstract:

This works introduces a comprehensive research experiment about the synthesis and characterization of nanoprobes for detecting reactive oxygen species (ROS). In this experiment, organic semiconducting polymer nanoprobes were prepared using nano-coprecipitation method. Those nanoprobes can be employed for ratiometric detection of ROS, using semiconductor polymer (PFODBT) as the reference and fluorescent molecules (IR775s) as responsive unit. Specifically, the content of ROS (ClO^- and ONOO^-) can be measured by calculating PFODBT-to-IR775s absorbance ratio. Moreover, based on the principle of fluorescence resonance energy transfer (FRET) between PFODBT and IR775s, the ratio of fluorescent emission of PFODBT/IR775s can also be used for fluorescence ratiometric detection of ROS. This experiment is comprehensive and feasible, and benefited for training the solid experimental skills and scientific research ability for students.

Key words: Analytical measurement, Nanoprobe, Fluorescence resonance energy transfer, Ratiometric detection, Reactive oxygen species

MSC2000:

Guosheng Song, Shiyi Liao, Youjuan Wang, Shuangyan Huan. Semiconducting Polymer Nanoprobe for Ratiometric Detection of Reactive Oxygen Species: A Comprehensive Experiment[J].University Chemistry, 2021, 36(8): 2011032.

Figures/Tables 11

References 14

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仪器名称	型号	生产厂家
紫外-可见吸收光谱仪	UV-2450	Shimadzu (日本)
荧光光谱仪	Fluoromax-4	Jobin Yvon (法国)
高速离心机	H2050R	湖南湘仪实验室仪器开发有限公司
旋转蒸发仪	RE301	郑州科达机械仪器设备公司
透射电子显微镜	F2100	日本电子株式会社生产
超声波清洗仪	KM5200DE	昆山美美超声仪器有限公司

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Semiconducting Polymer Nanoprobe for Ratiometric Detection of Reactive Oxygen Species: A Comprehensive Experiment

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