University Chemistry ›› 2021, Vol. 36 ›› Issue (9): 2011055.doi: 10.3866/PKU.DXHX202011055

Special Issue: 分析化学课程与实验教学

• Chemistry Laboratory • Previous Articles     Next Articles

Design of Experiment for Determination of Caffeine Content by Ultra Performance Convergence Chromatography

Yifan Xie1, Liyun Yao1,2,*(), Zhonghong Yan2, Yuxing Cai1, Yujie Jin1, Ning Li1   

  1. 1 Basic Medical Experimental Teaching Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
    2 Medicinal Chemistry and Bioinformatics Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
  • Received:2020-11-25 Accepted:2021-12-28 Published:2021-01-15
  • Contact: Liyun Yao E-mail:yaoliyun517@163.com

Abstract:

An experiment of instrumental analysis for determination of caffeine content in beverages by Ultra Performance Convergence Chromatography (UPC2) was designed. The caffeine in three beverages including coke, green tea and oolong tea was extracted and diluted by methanol. Then caffeine concentrations in beverages were measured by UPC2. Through this experiment, students could not only consolidate several laboratory skills such as weighing, constant volume and liquid transfer, but also master the basic theory, operation and data processing of UPC2. This experiment integrates the new chromatography into basic laboratory teaching, which can broaden students' scientific horizons. It is conductive to cultivating students' innovative spirit and practical ability.

Key words: UPC2, Caffeine, Content determination, Laboratory teaching

MSC2000: 

  • G64