紫外-可见分光光度法测定含铜废水中的铜离子

doi:10.3866/PKU.DXHX202101012

Abstract

Abstract:

A teaching experiment for determining copper(Ⅱ) ions in copper-containing sewage by UV-Vis spectrophotometry is designed. The experiment is performed based on the Fenton reaction principle, where the presence of copper(Ⅱ) ions in the assay solution catalyzes the decomposition of hydrogen peroxide to produce hydroxyl radicals. The latter oxidizes the colorless 3, 3', 5, 5'-tetramethylbenzidine (TMB) in the solution to the yellow TMB²⁺, which can be used for quantitative analysis copper(Ⅱ) ions in copper-containing sewage by measuring the absorbance of TMB²⁺ at 452 nm. With easy operation, short measurement time, excellent stability, eco-friendliness, this experiment conforms to the basic requirements of undergraduate laboratory teaching.

Key words: Fenton reaction, Cu(Ⅱ) detection, Ultraviolet-visible spectrophotometry, Undergraduate teaching experiment

MSC2000:

Ziheng Zhang, Ran Hu, Yijin Ma, Xingrong Sun, Siwei Ma, Ren Zhu, Qiue Cao, Chuanhua Zhou. An Experiment for Determining Copper(Ⅱ) Ions in Copper-Containing Sewage by UV-Vis Spectrophotometry[J].University Chemistry, 2021, 36(9): 2101012.

Figures/Tables 9

References 19

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编号	组别	开始时间	结束时间
空白	Blank	00:00	30:00
1	Std.1	01:00	31:00
2	Std.2	02:00	32:00
3	Std.3	03:00	33:00
4	Std.4	04:00	34:00
5	Std.5	05:00	35:00
6	Samp.1	06:00	36:00
7	Samp.2	07:00	37:00
8	Samp.3	08:00	38:00

Std. No.	c_标/(μmol·L^-1)	A_{452 nm}
1	0.25	0.342
2	0.50	0.445
3	0.75	0.534
4	1.00	0.622
5	1.25	0.708

Samp. No.	A_{452 nm}	c_样/(μmol·L^-1)
1	0.471	0.587
2	0.476	0.601
3	0.465	0.571

${\bar c}$/(μmol·L^-1)	RSD	原浓度/(μmol·L^-1)	相对误差
0.586	1.50%	5.86	2.33%

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An Experiment for Determining Copper(Ⅱ) Ions in Copper-Containing Sewage by UV-Vis Spectrophotometry

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