University Chemistry ›› 2022, Vol. 37 ›› Issue (7): 2111033.doi: 10.3866/PKU.DXHX202111033

• Chemistry Laboratory • Previous Articles     Next Articles

Fabrication of 3D N-Doped Carbon Nanospheres and Their Application in Electrochemical Sensing: A Comprehensive Synthetic and Analytical Experiment

Xiaoyi Yan1,2,*(), Qiang Ma1,2, Shigang Wei1,2, Zhiquan Zhang1, Yupeng Guo1,2   

  1. 1 College of Chemistry, Jilin University, Changchun 130012, China
    2 National Experimental Teaching Demonstrating Center of Chemistry, Jilin University, Changchun 130012, China
  • Received:2021-11-10 Accepted:2021-12-13 Published:2022-02-10
  • Contact: Xiaoyi Yan


A comprehensive exploratory chemical experiment is designed to introduce carbon-based bioelectrochemical materials into undergraduate experimental teaching. This experiment consists of preparing three-dimensional N-doped carbon nanospheres (3D-N-CB) and applying them in the electrochemical detection of glutathione. First, 3D-N-CB was synthesized via one-pot pyrolysis using melamine and carbon black as the raw materials. Subsequently, the bioelectrochemical sensing performance of 3D-N-CB was studied using various electrochemical techniques, and glutathione detection was successfully achieved under optimal conditions. This experiment can fully embody the scientific research thinking mode of synthesis-characterization-application. And it can cultivate the ability of undergraduate students to innovate independently in scientific research. Therefore, the experiment can effectively improve the basic experimental skills and comprehensive application ability of undergraduates.

Key words: Bioelectrochemical sensor, Comprehensive exploratory experiment, N-doped carbon nanospheres, Glutathione


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