University Chemistry ›› 2015, Vol. 30 ›› Issue (2): 50-55.doi: 10.3866/pku.DXHX20150250

• Chemistry Laboratory • Previous Articles     Next Articles

Application of a Low-cost Optical Tweezers Setup in Fluorescence Quantitative Detection

Tang Hongwu, Cao Di, Li Chengyu, Pang Daiwen   

  1. College of Chemistry and Molecular Sciences, Key Laboratory of Analytical Chemistry for Biology and Medicine(Ministry of Education), Wuhan University, Wuhan 430072, Hubei, China
  • Published:2015-04-25

Abstract:

A simple and feasible optical tweezers setup based on a domestic upright fluorescence microscope was constructed, and it was successfully applied for two-dimensional trapping of a single microsphere. By combining this setup with fluorescence detection and using fluorescent quantum dots (QDs) with emission wavelength 605nm as the tags, a quantitative method with high sensitivity and selectivity was developed when single microspheres were applied to enrich the target molecules, and as a proof-of-concept study the quantitative analysis of human papillomavirus (HPV) 16 DNA was realized.

Key words: Optical tweezer, Fluorescence, Quantum dots, DNA, Quantitative analysis