两种比色法检测水体中微量氨含量的比较研究

doi:10.3866/PKU.DXHX201807034

University Chemistry ›› 2019, Vol. 34 ›› Issue (3): 36-41.doi: 10.3866/PKU.DXHX201807034

• Chemistry Laboratory • Previous Articles Next Articles

Comparison of Two Colorimetric Methods for the Determination of Trace Ammonia in Water

Jiamin WANG¹,Han LI¹,Wenkai ZHANG²,Chengshang LIU³,Baoliang LIU¹,Huayu SUN¹,Qikun ZHANG^1,*()

¹ Chemical National Experimental Teaching Demonstration Center, College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Ji'nan 250014, P. R. China
² Handan No.1 High School, Handan 056001, Hebei Province, P. R. China
³ Affiliated Secondary School of Shandong Normal University, Ji'nan 250014, P. R. China

Received:2018-07-26 Accepted:2018-08-19 Published:2019-03-09
Contact: Qikun ZHANG E-mail:zhangqk@sdnu.edu.cn
Supported by:
山东师范大学本科生科研基金项目(2017BKSKY49);山东师范大学本科生科研基金项目(2018BKSKY54);大学生创新训练计划项目(20181501);山东师范大学教学改革项目：工程教育认证背景下化学工程与工艺专业建设与教学改革新探索

Abstract

Abstract:

The level of ammonia nitrogen source is one indicator of eutrophication of lakes and reservoirs. Currently, indigo phenol blue spectrophotometry and Nash reagent spectrophotometry have been adopted to detect the NH₃-N content in national standard of the People's Republic of China. To cultivate college students' innovative practice, scientific research consciousness and experimental hands-on ability, the ammonia nitrogen concentration in the water samples of three famous springs (Baotu Spring, Heihu spring, Wulong pool), Daming Lake, Dongchang Lake and Haihe river was tested. Taking the drinking water from Shandong Normal University for comparison, the differences between the two methods were studied. More importantly, the students learned from the success and failure in the testing process that they used the colorimetric method to detect and analyze samples. The correlation coefficients of the standard curves of the two methods reached up to 0.9999 through continuous improvement of the detection scheme. In the experimental operation, different experimental results have been obtained due to the variation of operation and manipulation (such as the concentration of chromogenic agent, the operation skill of the standard solution and the length of the time after the mixing of each solvent etc.). This paper analyzes the causes of the outlier in the experimental results, and discusses the difference of two detection methods.

Key words: Indophenol blue spectrophotometry, Nessler's reagent method, Colorimetric method, Ammonia nitrogen concentration, Absorbance

MSC2000:

Jiamin WANG,Han LI,Wenkai ZHANG,Chengshang LIU,Baoliang LIU,Huayu SUN,Qikun ZHANG. Comparison of Two Colorimetric Methods for the Determination of Trace Ammonia in Water[J].University Chemistry, 2019, 34(3): 36-41.

Figures/Tables 5

References 12

1	赵倩. 1997-2007年上海市青浦区水体氮素含量时空变化特征及驱动因子分析[D].上海:复旦大学, 2009.
2	卢可.氨氮和COD测定方法的改进及研究[D].安庆:安庆师范大学, 2017.
3	张铃松; 王业耀; 孟凡生; 刘廷良; 马广文; 周岳溪; 刘允. 环境科学与技术, 2013, 10 (43)
4	徐烜峰; 李维红; 边磊; 关玲; 李恩敬; 张奇涵. 大学化学, 2018, 33 (4), 41.
5	封跃鹏; 邱赫男; 孙自杰. 环境科学与技术, 2016, 39 (S2), 348.
6	钱林平; 李进之; 郑耿锋; 沈建中. 大学化学, 2018, 33 (2), 60.
7	赵艳芳; 徐鲁斌. 大学化学, 2017, 32 (12), 69. doi: 10.3866/PKU.DXHX201706027
8	GB 3838-2002.地表水环境质量标准.北京:中国标准出版社, 2002.
9	QX/T 217-2013.大气中氨(铵)测定:靛酚蓝分光光度法.北京:中国标准出版社, 2014.
10	HJ 535-2009.水质氨氮的测定纳氏试剂分光光度法.北京:中国环境科学出版社, 2010.
11	GB/T 18204.2-2014.公共场所卫生检验方法第2部分:化学污染物.北京:中国标准出版社, 2014.
12	GB 5749-2006.生活饮用水卫生标准.北京:中国标准出版社, 2007.

管号	氨标准工作液/mL	吸收液/mL	氨含量/咫	氨浓度/(mg.L^-1)
0	0.00	10.00	0.00	0.00
1	0.40	9.60	0.40	0.04
2	0.60	9.40	0.60	0.06
3	1.00	9.00	1.00	0.10
4	2.00	8.00	2.00	0.20
5	5.00	5.00	5.00	0.50
6	7.00	3.00	7.00	0.70

水样名称	第一次吸光度	第二次吸光度	平均值	氨浓度/(mg-L^-1)
大明湖	0.047	0.047	0.047	0.06
黑虎泉	0.057	0.050	0.054	0.07
趵突泉	0.055	0.046	0.051	0.07
五龙潭	0.058	0.044	0.051	0.07
东昌湖	0.129	0.147	0.138	0.19
海河	0.187	0.193	0.190	0.26
自来水	0.073	0.057	0.065	0.09

水样名称	第一次吸光度	第二次吸光度	平均值	氨含量/(mg-L^-1)
大明湖	0.056	0.053	0.0545	-0.47
黑虎泉	0.045	0.049	0.047	-1.00
趵突泉	0.050	0.048	0.049	-0.86
五龙潭	0.051	0.052	0.0515	-0.68
东昌湖	0.071	0.076	0.0735	0.90
海河	0.084	0.080	0.082	1.50
自来水	0.045	0.046	0.0455	-1.11

[1]	Yinyun Lü, Yuhua Weng, Rui Pan, Chunyan Zhang, Zhiqiang Dong, Xiaoqing Ouyang, Chanzi Ruan, Zhenling Xu, Shunü Peng, Yanping Ren. The Flexible Application of QR Code in the Laboratory Teaching, Laboratory Instrument Management and Academic Report [J]. University Chemistry, 0, (): 0-0.
[2]	Laiying Zhang, Ruyue Lan, Zhihong Liu, Weitai Wu. Aqueous Synthesis and Characterization of Temperature-Responsive Polymer Microgels: An Introduction of a Comprehensive Chemical Experiment [J]. University Chemistry, 0, (): 0-0.
[3]	Xiaoliang Liu, Yangqiu Liu, Ming Lei. A Web-Based Point Group Teaching Platform (PGLite) in Structural Chemistry Course [J]. University Chemistry, 0, (): 0-0.
[4]	Ling Xu, Hengwei Wei, Lingling Wei, Yi Ma, Xiaoming Wang, Huan Jiao. Exploration and Practice of Course Ideology and Politics Education in Basic Inorganic Chemistry Laboratory: Taking Preparation of Graphene Oxide as an Example [J]. University Chemistry, 0, (): 0-0.
[5]	Xiufang Xu. Quantum Chemistry Calculation Experiment on Activation Energy for Isomerization of HCN and HNC [J]. University Chemistry, 0, (): 0-0.
[6]	Tianjiao Fan, Qizhi Yao, Gu Jin. Exploration and Practice of Undergraduate Self-Design Experiments: Based on the Disposal and Analysis of Wastewater Containing Chromium Ion [J]. University Chemistry, 0, (): 0-0.
[7]	Hongyu Liu, Sihao Yuan, Gu Jin. Study on the Colour Reaction System of Iodine and Water-Soluble Polymer [J]. University Chemistry, 0, (): 0-0.
[8]	Yan Deng. Iodometry-Based Standardization of Iodine Value of Nut Oil: An Introduction of a Comprehensive Analytical Chemistry Experiment [J]. University Chemistry, 0, (): 0-0.
[9]	Demei Tian. Research into the Instruction on Preparation and Purification of Aspirin [J]. University Chemistry, 0, (): 0-0.
[10]	Yinlu Sun, Hongyue Jiang, Ying Xiong. Exploration and Practice of Personnel Training in Analytical Chemistry on the Background of “Course Ideology and Politics”: Take Chromatography Teaching as an Example [J]. University Chemistry, 0, (): 0-0.
[11]	Rong Wei, Xue Wang, Shuihuan Yu, Zhekan Yao, Zhiwei Miao. Synthesis of Cyclotriphosphonitrile Sulfonate Flame Retardant and Application of Flame Retardant Polycarbonate: Undergraduate “Scientific Research Feedback Teaching” Comprehensive Experiment in Nankai University [J]. University Chemistry, 0, (): 0-0.
[12]	Shasha Liu, Huixiang Li, Jie Lei. Study on the Effect of Appearance of the Primary Standard on Standardizing the Standard Solution: Borax as an Example [J]. University Chemistry, 0, (): 0-0.
[13]	Xiaoshuang Ma, Chengbin Zheng. The Transition from Quantity to Quality: The Exploration and Reform on the International Training Scheme for Top Chemistry Undergraduate Students at the Stage of “Top-notch Undergraduate Training Program 2.0” [J]. University Chemistry, 0, (): 0-0.
[14]	Yongfen Tong, Zhimin Huang, Qiuju Xing. Reformation and Practice of Organic Chemistry Curriculum for Simultaneous Cultivation of Ability and Morality [J]. University Chemistry, 0, (): 0-0.
[15]	Fuxian Wan, Zhentao Wang, Jigang Gao, Ying Li, Changcheng Li, Lin Jiang, Shiyun Ai, Lili Zhang. The Construction and Practice of Ideological and Political Teaching Model of Agricultural Organic Chemistry Course under the Idea of Building Morality and Cultivating People [J]. University Chemistry, 0, (): 0-0.

Comparison of Two Colorimetric Methods for the Determination of Trace Ammonia in Water

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 12

Related Articles 15

Metrics

Recommended 0