University Chemistry ›› 2020, Vol. 35 ›› Issue (4): 119-124.doi: 10.3866/PKU.DXHX201911062

Special Issue: 全国大学生化学实验创新设计竞赛

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Enzymatic Synthesis of Glycerol Monolaurate Catalyzed by Lipase Novozym 435

Zijun Yan, Xin Zhang, Zuoao Wu, Kaiqun Wu(), Yuliang Wang   

  • Received:2019-11-27 Accepted:2019-12-16 Published:2020-02-12
  • Contact: Kaiqun Wu


The potential of biocatalysis has been recognized as an efficient and green tool for modern organic synthesis due to its high selectivity, mild reaction conditions and sustainability. The enzyme-catalyzed synthesis of glycerol monolaurate were investigated in this study. The optimized reaction takes place with a 1:3.5 molar ratio of lauric acid to glycerol in the presence of 5% enzyme catalyst at 52℃ for 80 min. The product with 90% purity was obtained in 47%-53% yield via several separation steps. This enzymatic synthesis shows high stability and good repeatability, and is a suitable organic chemistry experiment for undergraduate laboratory teaching.

Key words: Lipase, Novozym 435, Glycerol monolaurate


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