响应面法在仪器分析实验教学中的应用——以响应面法优化HPLC测定饮料苯甲酸的条件为例

doi:10.3866/PKU.DXHX202011040

Abstract

Abstract:

The instrumental analysis course is one of the professional basic courses that combine theory and experiment. In order to improve students' scientific research and innovation ability, "the application of response surface method to the optimization of HPLC conditions for determination of benzoic acid in beverages" is taken as an example. Based on the current situation of the experimental course of "instrumental analysis", the data processing software used in response surface analysis is studied in experimental teaching and helps students understand and be proficient in the experimental design and software of response surface methodology.

Key words: Response surface, Instrumental analysis, Experimental teaching

MSC2000:

Jialiexi·Manafu, Janaer·Tuerxun, Yue Wan, Fudong Ma. Application of Response Surface Method in the Teaching of Instrumental Analysis Experiment: Taking the Optimization of HPLC Conditions for Determination of Benzoic Acid in Beverages as an Example[J].University Chemistry, 2021, 36(8): 2011040.

Figures/Tables 4

References 11

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因素	水平
因素	−1	0	1
A/min	5	10	15
B/%	10	12.5	15
C	6	7	8

试验号	A	B	C	Y/(mg∙kg⁻¹)
1	−1	0	−1	135
2	0	−1	1	138
3	0	0	0	140
4	−1	−1	0	135
5	1	0	1	138
6	1	1	0	130
7	0	0	0	140
8	1	0	−1	136
9	1	−1	0	135
10	0	0	0	140
11	−1	0	1	138
12	0	1	1	137
13	0	−1	−1	136
14	0	0	0	140
15	−1	1	0	137
16	0	1	−1	135
17	0	0	0	140

方差来源	平方和	自由度	均方	F值	P值	显著性
模型	106.69	9	11.85	10.06	0.0030	**
A	4.50	1	4.50	3.82	0.0916
B	3.12	1	3.12	2.65	0.1475
C	10.13	1	10.13	8.59	0.022	*
AB	12.25	1	12.25	10.39	0.0146	*
AC	0.25	1	0.25	0.21	0.6591
BC	1.421 × 10⁻¹⁴	1	1.421 × 10⁻¹⁴	1.206 × 10⁻¹⁴	1.0000
A²	31.84	1	31.84	27.02	0.0013	**
B²	37.89	1	37.89	32.15	0.0008	***
C²	1.05	1	1.05	0.89	0.3761
残差	8.25	7	1.18
失拟项	8.25	3	2.75	1.47	0.2504
纯误差	0.000	4	0.000
R² = 0.9282

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Application of Response Surface Method in the Teaching of Instrumental Analysis Experiment: Taking the Optimization of HPLC Conditions for Determination of Benzoic Acid in Beverages as an Example

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