University Chemistry ›› 2018, Vol. 33 ›› Issue (6): 33-37.doi: 10.3866/PKU.DXHX201801033

• Chemistry Laboratory • Previous Articles     Next Articles

Recommending an Chromatographic Analysis Experiment: Determination of the Content of Dexamethasone Acetate in Mixed Cream by High Performance Liquid Chromatography (HPLC)

Xiaohui GAO1,Haoran LIU2,*()   

  1. 1 College of Pharmacy, Changsha Medical University, Changsha 410219, P. R. China
    2 College of Chemistry and Chemical Engineering, Hu'nan University, Changsha 410082, P. R. China
  • Received:2018-01-22 Published:2018-06-01
  • Contact: Haoran LIU E-mail:tom8833@hnu.edu.com
  • Supported by:
    湖南省普通高校“十三五”专业综合改革试点项目(湘教通[2016]276);长沙医学院校级教改课题(长医教[2017]44号);湖南大学“校企沟通创新创业基地”基金

Abstract:

This paper recommended the experiment of "Determination of the content of dexamethasone acetate in mixed cream by high performance liquid chromatography", in this experiment several experimental skills such as weighing, hot water bath, vortex, ice bath, filtration, liquid transfer and other sample pretreatment skills are included, and UV spectrophotometer and HPLC are used, as well as the methods of quantitative measurement and data processing are requested to get. This experiment can fully promote the students' experimental skills, enhance the ability for practical application, and raise the preliminary scientific research thinking.

Key words: Recommend, Dexamethasone acetate, HPLC, Ice bath, Cream

MSC2000: 

  • G64