C18反相色谱固定相的制备及性能评价——介绍一个仪器分析拓展性实验

doi:10.3866/PKU.DXHX201810011

Abstract

Abstract:

An extended instrumental analysis experiment "preparation and performance evaluation of C₁₈ stationary phase for reversed-phase chromatography" is recommended for undergraduates. The experiment includes the synthesis, characterization, preparation and properties of the stationary phase. The knowledge of organic synthesis, physical chemistry and instrumental analysis is covered in the experiment. With the practice of the experiment, the typical instrumental analysis experiment is extended. It is helpful for students to cultivate the ability of applying knowledge comprehensively and exercise the ability of scientific research.

Key words: Instrumental analysis experiment, Chromatography, Stationary phase preparation, Performance evaluation

MSC2000:

Lihong NIU,Yun ZHOU,Ying YU,Yuan ZHANG,Bilun WU,Weiqi MAI. Preparation and Performance Evaluation of C₁₈Stationary Phase for Reversed-Phase Chromatography: An Extended Instrumental Analysis Experiment[J].University Chemistry, 2019, 34(5): 46-50.

样品	保留时间t_R/min		半峰宽W_h_/2/min		理论塔板数N/m		拖尾因子T
样品	商品柱	自制柱	商品柱	自制柱	商品柱	自制柱	商品柱	自制柱
苯	3.31	3.44	0.123	0.171	3999	2245	1.872	1.940
甲苯	4.11	4.49	0.151	0.191	4119	3058	0.944	1.986
联苯	6.37	7.51	0.190	0.262	6218	4558	1.003	2.215

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Preparation and Performance Evaluation of C₁₈Stationary Phase for Reversed-Phase Chromatography: An Extended Instrumental Analysis Experiment

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Preparation and Performance Evaluation of C₁₈Stationary Phase for Reversed-Phase Chromatography: An Extended Instrumental Analysis Experiment