论如何通过设计性实验提升学生的综合创新能力——以分光光度法同时测定维生素C和维生素E为例

doi:10.3866/PKU.DXHX201908025

University Chemistry ›› 2020, Vol. 35 ›› Issue (9): 76-82.doi: 10.3866/PKU.DXHX201908025

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Discussions on How to Improve the Comprehensive Innovation Abilities of Students by Designed Experiments: An Example of Simultaneous Determination of Vitamin C and E by Spectrophotometry

Jingyu Wang¹^,*(), Heng Wang¹, Xiong Hui¹, Zhifei Chen¹, Hong Yuan²

¹ School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, P. R. China
² National Demonstration Center for Experimental Chemistry Education, College of Chemistry (Central China Normal University), Wuhan 430079, P. R. China

Received:2019-08-17 Accepted:2019-09-24 Published:2019-10-31
Contact: Jingyu Wang E-mail:wangjingyu@hust.edu.cn

Abstract

Abstract:

Simultaneous determination of vitamin C and vitamin E by spectrophotometry is a typical experiment of simultaneous determination of multiple components. However, the current experimental method has a large relative error (13.7%) in the determination of vitamin C. In this paper, according to the molecular structural characteristics of vitamin C and vitamin E, we studied the factors affecting the measurement accuracy of vitamin C and improve the experimental method by optimization pf design, and the error is reduced to 1.98%. Results proved that the improved method achieved higher accuracy, better reproducibility, more reliable results and better teaching responses. Through this designed experiment, the students are guided from many aspects such as aiming at practical problems and using theoretical knowledge to optimize the experimental scheme, therefore their comprehensive innovation abilities are improved.

Key words: Simultaneous determination, Vitamin C, Vitamin E, Spectrophotometry, Comprehensive innovation ability

MSC2000:

Jingyu Wang, Heng Wang, Xiong Hui, Zhifei Chen, Hong Yuan. Discussions on How to Improve the Comprehensive Innovation Abilities of Students by Designed Experiments: An Example of Simultaneous Determination of Vitamin C and E by Spectrophotometry[J].University Chemistry, 2020, 35(9): 76-82.

Figures/Tables 6

References 12

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测试条件	标准曲线
	维生素C		维生素E
	λ₁	λ₂	λ₁	λ₂
无水乙醇	y = 0.0928x + 0.0265 (R² = 0.9817)	y = 0.0027x + 0.0646 (R² = 0.5842)	y = 0.0024x − 0.0001 (R² = 0.9900)	y = 0.0143x + 0.0002 (R² = 0.9999)
无水乙醇加适量稀硫酸	y = 0.1075x + 0.0124 (R² = 0.9995)	y = −0.0006x + 0.0054 (R² = 0.6452)	y = 0.0032x − 0.0198 (R² = 0.9876)	y = 0.0143x − 0.0031 (R² = 0.9999)

溶剂	λ₁处吸光度	λ₂处吸光度	测试c_C/(mg∙L⁻¹)	测试c_E/(mg∙L⁻¹)	c_C相对误差/%	c_E相对误差/%
无水乙醇	0.3672	0.2398	3.54	16.10	13.65	40.01
无水乙醇加适量稀硫酸	0.4866	0.1634	4.18	11.60	1.98	0.89

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Discussions on How to Improve the Comprehensive Innovation Abilities of Students by Designed Experiments: An Example of Simultaneous Determination of Vitamin C and E by Spectrophotometry

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