University Chemistry ›› 2020, Vol. 35 ›› Issue (9): 76-82.doi: 10.3866/PKU.DXHX201908025

• Chemistry Laboratory • Previous Articles     Next Articles

Discussions on How to Improve the Comprehensive Innovation Abilities of Students by Designed Experiments: An Example of Simultaneous Determination of Vitamin C and E by Spectrophotometry

Jingyu Wang1,*(), Heng Wang1, Xiong Hui1, Zhifei Chen1, Hong Yuan2   

  1. 1 School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, P. R. China
    2 National Demonstration Center for Experimental Chemistry Education, College of Chemistry (Central China Normal University), Wuhan 430079, P. R. China
  • Received:2019-08-17 Accepted:2019-09-24 Published:2019-10-31
  • Contact: Jingyu Wang


Simultaneous determination of vitamin C and vitamin E by spectrophotometry is a typical experiment of simultaneous determination of multiple components. However, the current experimental method has a large relative error (13.7%) in the determination of vitamin C. In this paper, according to the molecular structural characteristics of vitamin C and vitamin E, we studied the factors affecting the measurement accuracy of vitamin C and improve the experimental method by optimization pf design, and the error is reduced to 1.98%. Results proved that the improved method achieved higher accuracy, better reproducibility, more reliable results and better teaching responses. Through this designed experiment, the students are guided from many aspects such as aiming at practical problems and using theoretical knowledge to optimize the experimental scheme, therefore their comprehensive innovation abilities are improved.

Key words: Simultaneous determination, Vitamin C, Vitamin E, Spectrophotometry, Comprehensive innovation ability


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