分光光度法测定铬黑T解离常数实验设计

孙瑞卿, 许紫婷, 陈珠灵, 林佳丽

Experimental Design on the Determination of Dissociation Constants of Eriochrome Black T by Spectrophotometry

Ruiqing Sun, Ziting Xu, Zhuling Chen, Jiali Lin

表5 不同波长处拟合方程及测定的pKa值

波长 拟合方程 pKa值 R2 (可决系数) 516 nm y = 0.661x + 3.85 pKa1 = 3.85 0.979 618 nm y = 1.24x + 3.94 pKa1 = 3.94 0.966 516 nm y = 0.441x + 6.35 pKa2 = 6.35 0.987 618 nm y = 0.644x + 6.41 pKa2 = 6.41 0.982 455 nm y = 1.29x + 11.57 pKa3 = 11.57 0.980 618 nm y = 1.07x + 11.43 pKa3 = 11.43 0.989