分光光度法测定铬黑T解离常数实验设计

孙瑞卿, 许紫婷, 陈珠灵, 林佳丽

Experimental Design on the Determination of Dissociation Constants of Eriochrome Black T by Spectrophotometry

Ruiqing Sun, Ziting Xu, Zhuling Chen, Jiali Lin

表6 不同波长处测定的pKa值及准确度

文献值 测定平均值 相对偏差 样品数n 标准差S F检验 F表 F计算 pKa1 = 3.9 516 nm，pKa1 = 3.85 ?1.38% 5 0.0342 6.26 2.29 618 nm，pKa1 = 3.94 1.13% 6 0.0518 pKa2 = 6.4 516 nm，pKa2 = 6.35 ?0.78% 6 0.0272 4.95 2.29 618 nm，pKa2 = 6.41 0.16% 7 0.0412 pKa3 = 11.5 455 nm，pKa3 = 11.57 0.61% 11 0.0511 3.14 2.79 618 nm，pKa3 = 11.43 ?0.61% 10 0.0306