理性改造光脱羧酶并用于反式脂肪酸选择性脱羧反应
曾森海, 鲍雨妍, 曹奡, 黄小强
Rational Evolution of a Fatty Acid Photodecarboxylase for Highly Selective Photodecarboxylation of
trans
Fatty Acid
Senhai Zeng, Yuyan Bao, Ao Cao, Xiaoqiang Huang
表4
粗酶浓度的测定
a
酶液
A
b
A
0
c
A − A
0
c
(FAD)/(mmol∙L
−1
)
c
(粗酶)/(mmol∙L
−1
)
WT-
Cv
FAP
0.742
0.647
0.095
0.0201
0.100
V453E-
Cv
FAP
0.764
0.647
0.117
0.0247
0.124