大学化学 >> 2020, Vol. 35 >> Issue (4): 132-136.doi: 10.3866/PKU.DXHX201911058

所属专题： 全国大学生化学实验创新设计竞赛

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电聚合制备分子印迹电极及其特异性表征

吴志铭, 张佳宁, 李仕菊, 许新华(), 刘梅川()   

  • 收稿日期:2019-11-26 录用日期:2019-12-16 发布日期:2020-02-25
  • 通讯作者: 许新华,刘梅川 E-mail:xxh01@tongji.edu.cn;liumc@tongji.edu.cn

Molecularly Imprinted Electrode Prepared by Electropolymerization Method and its Characterization

Zhiming Wu, Jianing Zhang, Shiju Li, Xinhua Xu(), Meichuan Liu()   

  • Received:2019-11-26 Accepted:2019-12-16 Published:2020-02-25
  • Contact: Xinhua Xu,Meichuan Liu E-mail:xxh01@tongji.edu.cn;liumc@tongji.edu.cn

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摘要：

本实验设计以邻苯二胺为功能单体，以双酚A为目标分子，采用简单快速的电聚合方法，制备了对双酚A具有特异性识别能力的分子印迹电极。采用差分脉冲伏安法和石英晶体微天平对印迹电极的特异识别性能进行表征。结果表明，印迹电极对双酚A表现出较高的特异性识别能力。与非印迹电极相比，识别能力提高一个数量级。同时，该电极对100倍浓度的阿特拉津、17β-雌二醇的信号响应仅为十分之一，表明该电极在复杂体系中具有良好的抗干扰能力。此外，该实验方案目标分子选择灵活，能制备针对不同目标分子的印迹电极，可满足自主设计实验的需求。通过该创新实验，可以有效帮助学生深入了解分子间的相互作用，体验在分子层次设计材料的过程。

关键词: 实验设计, 分子印迹, 电极, 电聚合法, 特异性

Abstract:

In this experiment, molecularly imprinting polymers (MIP) electrodes with specific recognition ability of bisphenol A (BPA) were prepared by electropolymerization method. O-phenylenediamine was used as the polymeric monomer and BPA as the target molecule. The MIP electrodes were characterized by differential pulse voltammetry and quartz crystal microbalance. Results showed that the electrodes had highly specific recognition ability for BPA. In comparison with the non-imprinting polymers electrode, the recognition ability of MIP was improved by one order of magnitude. 100-fold concentration of atrazine or 17β-estradiol only altered the signal by one-tenth, indicating high selectivity of BPA-MIP electrode in complex matrix. In addition, this experimental design allows flexible target selection. Therefore, after changing the target molecules, the similar MIP electrode could be also successfully prepared by the same method. This experiment allows students to design experiments by themselves. And students can gain an in-depth understanding of the interaction between molecules, and experience the process of designing materials at the molecular level.

Key words: Experimental design, Molecularly imprinting, Electrodes, Electropolymerization, Specificity