大学化学 >> 2020, Vol. 35 >> Issue (6): 110-116.doi: 10.3866/PKU.DXHX201911025

所属专题: 大学生实践活动

  上一篇    

一种基于智能手机可视化多色荧光检测碱性磷酸酶活性的方法——推荐一个本科生科研训练项目

秦雨欣, 林天然(), 李金莹, 覃思媛, 侯丽()   

  • 收稿日期:2019-11-18 录用日期:2020-02-06 发布日期:2020-02-27
  • 通讯作者: 林天然,侯丽 E-mail:tianran@gxnu.edu.cn;houli@gxnu.edu.cn
  • 基金资助:
    2018–2020年度广西本科高校特色专业及实验实训教学基地(中心)建设项目(桂教高教[2018]52号);广西师范大学化学与药学学院教学改革研究项目(2019XJGB31);广西自然科学基金项目(2018GXNSFBA050053)

A Visual Multicolor Fluorescent Assay for Alkaline Phosphatase Activity Based on a Smartphone: Recommendation of an Undergraduate Research-Training Program

Yuxin Qin, Tianran Lin(), Jinying Li, Siyuan Qin, Li Hou()   

  • Received:2019-11-18 Accepted:2020-02-06 Published:2020-02-27
  • Contact: Tianran Lin,Li Hou E-mail:tianran@gxnu.edu.cn;houli@gxnu.edu.cn

摘要:

介绍一个本科生科研训练项目。本项目合成了具有荧光性能的铜金属有机框架材料,并用于构建一种基于智能手机可视化多色荧光检测碱性磷酸酶活性的方法。该方法可应用于血清中碱性磷酸酶含量的可视化检测。本科生在该科研项目训练中得到了充分的锻炼。本文详细介绍了本科生在该项目选题、实验方案设计、实验过程、取得的成果以及经验收获等。

关键词: 科研训练计划, 碱性磷酸酶, 多色荧光传感器, 可视化检测, 手机应用程序

Abstract:

In this program, a copper metal-organic framework material with fluorescence performance was synthesized and used to construct a multicolor fluorescent assay for alkaline phosphatase activity based on a smartphone. This assay was applied to the visual detection of alkaline phosphatase content in serum. Undergraduates have achieved the fullest training and knowledge during this program. This paper introduces in detail the topic selection, experimental scheme design, experimental process, achievements and experience gained by students in this project.

Key words: Research-training program, Alkaline phosphatase, Multicolor fluorescent biosensor, Visual detection, Mobile applications