大学化学 >> 2024, Vol. 39 >> Issue (8): 230-240.doi: 10.3866/PKU.DXHX202312062

化学实验 上一篇    下一篇

拉曼散射光谱法实验教学设计与实践

彭微, 温宝英, 李华敏, 王翊如, 李剑锋   

  1. 厦门大学化学化工学院化学系, 化学国家级实验教学示范中心(厦门大学), 福建 厦门 361005
  • 收稿日期:2023-12-19 修回日期:2024-02-26 发布日期:2024-03-08
  • 通讯作者: 王翊如, 李剑锋 E-mail:yrwang@xmu.edu.cn;Li@xmu.edu.cn
  • 基金资助:
    2022年度基础学科拔尖学生培养计划2.0(20222111)

Exploration and Practice on Raman Scattering Spectroscopy Experimental Teaching

Wei Peng, Baoying Wen, Huamin Li, Yiru Wang, Jianfeng Li   

  1. College of Chemistry and Chemical Engineering, Xiamen University, National Experimental Teaching Demonstration Center of Chemistry (Xiamen University), Xiamen 361005, Fujian Province, China
  • Received:2023-12-19 Revised:2024-02-26 Published:2024-03-08
  • Contact: Yiru Wang, Jianfeng Li E-mail:yrwang@xmu.edu.cn;Li@xmu.edu.cn

摘要: 拉曼光谱是一种无损的分析技术,能够提供物质的结构和成分信息,已被广泛应用于化学、生物学和材料科学等领域的分析和研究中。经过三年的教学,拉曼散射光谱法实验的教学设计已获得诸多实践经验。该实验项目分别设计了定性分析、定量分析和痕量分析三部分内容,从生活中常见的真假翡翠的辨别入手,激发与培养学生自主学习拉曼光谱相关知识的兴趣和热情。通过对便携式拉曼光谱仪的使用让学生理解仪器的结构和功能,掌握拉曼光谱法和表面增强拉曼光谱法的基本原理。经过本实验项目的学习,学生能够正确使用拉曼光谱仪测试固体样品、液体样品和痕量物质,为掌握相关的光谱学知识奠定良好的基础。

关键词: 拉曼散射光谱法, 表面增强拉曼光谱法, 定性分析, 定量分析, 拉曼光谱仪

Abstract: Raman spectroscopy, a non-destructive analytical technique, provides information about the structure and composition of substances. It has been widely applied in the fields of chemistry, biology, and materials science for analysis and research. Through three years of pedagogical development, our instructional design for Raman scattering spectroscopy experiment has accumulated considerable practical experience. This experimental course is structured around three segments: qualitative, quantitative, and trace analysis, initiating with the intriguing task of distinguishing between authentic and counterfeit jadeite—a familiar challenge that ignites students’ curiosity and eagerness to delve into Raman spectroscopy. Utilizing portable Raman spectrometer enables students to gain an understanding of the instrument's construction and functionality, while mastering the fundamental concepts of Raman spectroscopy and its surface-enhanced variant. This educational venture equips students with the proficiency to adeptly conduct Raman spectroscopic analyses on solid and liquid samples, as well as trace substances, thus establishing a solid foundation for their mastery of related spectroscopy analysis courses.

Key words: Raman scattering spectroscopy, Surface-enhanced Raman spectroscopy, Qualitative analysis, Quantitative analysis, Raman spectrometer