大学化学 >> 2024, Vol. 39 >> Issue (8): 295-305.doi: 10.3866/PKU.DXHX202401016

化学实验 上一篇    下一篇

链霉素分子印迹电化学传感器的制备及应用——推荐一个分析化学综合实验

冯佳蓉, 段烨堦, 楚楚, 谢德珍, 曹秋娥, 刘鹏   

  1. 云南大学化学科学与工程学院, 化学化工国家级实验教学示范中心(云南大学), 昆明 650091
  • 收稿日期:2024-01-05 修回日期:2024-03-11 发布日期:2024-04-12
  • 通讯作者: 刘鹏 E-mail:pliu@ynu.edu.cn
  • 基金资助:
    云南大学2023年度教育教学改革研究项目(2023Y44)；云南省大学生创新创业训练项目(S202310673227)

Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment

Jiarong Feng, Yejie Duan, Chu Chu, Dezhen Xie, Qiu'e Cao, Peng Liu   

  1. School of Chemical and Technology, Yunnan University, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Yunnan University), Kunming 650091, China
  • Received:2024-01-05 Revised:2024-03-11 Published:2024-04-12
  • Contact: Peng Liu E-mail:pliu@ynu.edu.cn

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摘要： 设计了一个将分子印迹技术引入到本科教学的探究性仪器分析综合实验。本实验首先通过电聚合法制备链霉素分子印迹电化学传感器(STR-MIM/GCE)，然后以循环伏安法和扫描电镜对传感器进行表征，再以铁氰化钾为探针，采用差示脉冲伏安法建立传感器测定链霉素的电化学分析方法，并以牛奶为实际样品进行检测。本实验涉及电极表面的修饰与表征、电化学分析方法的建立与应用，有助于拓宽学生思维，激发科研兴趣，同时实验所用试剂价廉易得，不涉及大型仪器设备，实验时长合理，适合本科教学。

关键词: 分子印迹技术, 电化学传感器, 链霉素

Abstract: This paper outlines a comprehensive, inquiry-based instrumental analysis experiment to integrate molecular imprinting technology into undergraduate education. The experiment commences with the fabrication of a streptomycin molecular imprinting electrochemical sensor (STR-MIM/GCE) via the electropolymerization method. Subsequent characterization of the sensor employs cyclic voltammetry and scanning electron microscopy. Utilizing potassium ferricyanide as a probe, a differential pulse voltammetry technique is developed for the electrochemical quantification of streptomycin, with milk serving as a real-world sample for testing. This experiment involves the modification and characterization of electrode surface, alongside the development and application of electrochemical analysis methods. The experiment uses readily available and cost-effective reagents, avoids the need for sophisticated instrumentation, and is designed with a duration suitable for undergraduate coursework.

Key words: Molecular imprinting technology, Electrochemical sensor, Streptomycin