大学化学 >> 2025, Vol. 40 >> Issue (2): 28-39.doi: 10.12461/PKU.DXHX202404020

所属专题： 教学型仪器的研制&改进与实验教学

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高效液相色谱仪器拆装实验设计与实践

肖华, 陈滔, 胡水, 李洁, 曾尊祥, 陈洪燕, 许先芳, 朱芳   

  1. 中山大学化学学院, 广州 510006
  • 收稿日期:2024-04-07 录用日期:2024-07-02 发布日期:2025-02-22
  • 通讯作者: 许先芳, 朱芳 E-mail:xuxianf@mail.sysu.edu.cn;ceszhuf@mail.sysu.edu.cn
  • 基金资助:
    中山大学教学质量与教学改革工程类项目

Design and Practice of High Performance Liquid Chromatography (HPLC) Disassembly and Assembly Experiment

Hua Xiao, Tao Chen, Shui Hu, Jie Li, Zunxiang Zeng, Hongyan Chen, Xianfang Xu, Fang Zhu   

  1. School of Chemistry, Sun Yat-sen University, Guangzhou 510006, China
  • Received:2024-04-07 Accepted:2024-07-02 Published:2025-02-22
  • Contact: Xianfang Xu, Fang Zhu E-mail:xuxianf@mail.sysu.edu.cn;ceszhuf@mail.sysu.edu.cn

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摘要： 分析仪器基础知识与操作技能是高校化学专业的重要教学内容，也是高校科研探究必备的基本素养。高效液相色谱仪作为一种重要的分析仪器，广泛应用于化学、生命科学、制药工业、环境科学、食品科学和石化分析等领域。为了进一步加深学生对液相色谱仪器结构与原理的理解，融会贯通，将理论应用于实践，本实验设计探究性仪器拆装实验教学内容，重新利用已报废的高效液相色谱仪，设计仪器拆装实验，引导学生打开仪器外壳，深度探索仪器内部“世界”。在实验过程中，有机融入课程思政教育，坚持以学生为中心，以探究为导向，增强学生独立思考与解决问题的综合能力，激发其探索未知、勇于创新的实践精神，培育其科技报国的家国情怀。

关键词: 液相色谱, 仪器拆装实验, 实验教学

Abstract: Understanding the structure and principles of analytical instruments is crucial in chemistry education and fundamental for scientific research in universities. High Performance Liquid Chromatography (HPLC) is extensively utilized in chemistry, life sciences, pharmaceuticals, environmental studies, food science, and petrochemical analysis. To deepen students’ comprehension, we designed an experimental disassembly and assembly using a decommissioned HPLC instrument. This experiment encourages students to dismantle the instrument, explore its internal workings, and apply theoretical knowledge to practical scenarios. Integrated with ideological and political education, the experiment centers on student-centered inquiry, enhancing their independent thinking, problem-solving abilities, and fostering a spirit of exploration and innovation. It aims to cultivate a sense of patriotism through dedication to scientific and technological advancement.

Key words: Liquid chromatography, Disassembly and assembly experiment, Laboratory teaching