大学化学 >> 2025, Vol. 40 >> Issue (6): 286-295.doi: 10.12461/PKU.DXHX202407059

化学实验 上一篇    下一篇

氨基酸的衍生及手性气相色谱分离创新实验

李润杰2, 刘航2, 王细胜2, 张万群1, 胡万群1, 杨凯平1, 周强1, 刘斯1, 朱平平2, 邵伟1   

  1. 1 化学国家级实验教学示范中心(中国科学技术大学), 合肥 230026;
    2 中国科学技术大学化学与材料科学学院, 合肥 230026
  • 收稿日期:2024-07-11 录用日期:2024-12-09 发布日期:2025-06-20
  • 通讯作者: 邵伟 E-mail:weishao@ustc.edu.cn
  • 基金资助:
    教育部首批虚拟教研室(大学化学实验课程群虚拟教研室)建设项目； 2024年中国科学技术大学研究生教研项目(2024ycjg02)； 2022年度安徽省质量工程项目(2022jyjxggyj023, 2022jyxm1822, 2022jyxm1827)

Innovative Experiments on Amino Acid Derivation and Chiral Gas Chromatographic Separation

Runjie Li2, Hang Liu2, Xisheng Wang2, Wanqun Zhang1, Wanqun Hu1, Kaiping Yang1, Qiang Zhou1, Si Liu1, Pingping Zhu2, Wei Shao1   

  1. 1 National Demonstration Center for Experimental Chemistry Education (University of Science and Technology of China), Hefei 230026, China;
    2 School of Chemistry and Materials Science, University of Science and Technology of China, Hefei 230026, China
  • Received:2024-07-11 Accepted:2024-12-09 Published:2025-06-20
  • Contact: SHAO Wei E-mail:weishao@ustc.edu.cn

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摘要： 氨基酸是蛋白质的重要组成单元。氨基酸的手性分子对映体往往有着类似的物理性质，但却具有完全不同的药理作用，因此，氨基酸的手性分离对于医药及生命科学有着极其重要的意义。手性气相色谱是氨基酸手性分离的重要手段。本实验使用氯甲酸乙酯(ECF)作为衍生化试剂，将氨基酸衍生化方法及手性分离引入到研究生“综合仪器分析实验”教学之中，可以让学生了解气相色谱从样品前处理、定性分析、定量检测到实验数据处理整个分析过程，在掌握基本操作的基础上进行探究，提升研究生的科研操作技能。

关键词: 氨基酸, 手性气相色谱, 氯甲酸乙酯, 衍生化

Abstract: Amino acids serve as fundamental constituents of proteins, whose chiral enantiomers exhibit distinct pharmacological activities despite sharing analogous physical properties. This biological significance necessitates precise chiral separation methodologies in pharmaceutical and life science research. The present experiment employs ethyl chloroformate (ECF) as a derivatization reagent to develop an instructional module for graduate-level analytical chemistry education. Our pedagogical design incorporates amino acid derivatization techniques coupled with chiral gas chromatographic separation into the Comprehensive Instrumental Analysis Laboratory curriculum. Through systematic training encompassing sample pretreatment, qualitative identification, quantitative determination, and data interpretation, this experimental protocol enables students to master the complete gas chromatographic analytical workflow. The exercise emphasizes operational standardization while encouraging exploratory analyses, thereby enhancing graduate students’ experimental proficiency in advanced analytical methodologies.

Key words: Amino acids, Chiral gas chromatography, Ethyl chloroformate, Derivatization