大学化学 >> 2025, Vol. 40 >> Issue (2): 121-129.doi: 10.12461/PKU.DXHX202409025

所属专题： 教学型仪器的研制&改进与实验教学

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暗场显微镜下的纳米颗粒识别与观测细胞膜上的扩散运动——推荐一个化学测量学实验<

孙元芳1,2,3, 张然1,2,3, 罗天1,2,3, 张欣1,2,3, 王翊如1,2, 黄腾翔1,2,3, 方宁1,2,3   

  1. 1 厦门大学化学化工学院, 固体表面物理化学国家重点实验室, 谱学分析与仪器教育部重点实验室, 福建 厦门 361005;
    2 化学国家级实验教学示范中心(厦门大学), 福建 厦门 361005;
    3 福建省能源材料科学与技术创新实验室, 福建 厦门 361102
  • 收稿日期:2024-09-25 录用日期:2024-10-22 发布日期:2025-02-22
  • 通讯作者: 方宁, 黄腾翔, 王翊如 E-mail:nfang@xmu.edu.cn;txhuang@xmu.edu.cn;yrwang@xmu.edu.cn
  • 基金资助:
    国家自然科学基金(32230063)；教育部基础学科拔尖学生培养计划2.0(20222111,20232023)；福建省本科高校教育教学研究重大项目(FBJY20230270)；中央高校基本科研业务费专项(20720220016)；教育部化学“101计划”——化学测量学实验课程建设项目；教育部第三批虚拟教研室建设试点——“101计划”化学测量学实验课程虚拟教研室

Identification of Nanoparticles and Observation of Diffusion on Cell Membranes Using Darkfield Microscopy: Recommending a Chemical Measurement Experiment

Yuanfang Sun1,2,3, Ran Zhang1,2,3, Tian Luo1,2,3, Xin Zhang1,2,3, Yiru Wang1,2, Tengxiang Huang1,2,3, Ning Fang1,2,3   

  1. 1 The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian Province, China;
    2 National Demonstration Center for Experimental Chemistry Education (Xiamen University), Xiamen 361005, Fujian Province, China;
    3 Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen 361102, Fujian Province, China
  • Received:2024-09-25 Accepted:2024-10-22 Published:2025-02-22
  • Contact: Ning Fang, Tengxiang Huang, Yiru Wang E-mail:nfang@xmu.edu.cn;txhuang@xmu.edu.cn;yrwang@xmu.edu.cn

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摘要： 单颗粒示踪是实现动态生物过程实时观测的重要技术突破，对于挖掘静态观察中所隐藏的机制尤为重要。随着光学显微成像的发展，商品化仪器能够轻松实现微观尺度下对于生物过程的实时观察。本实验采用纳米金、银材料作为成像探针，探讨了等离激元纳米材料的尺寸效应；并设计活细胞成像实验，通过观察金纳米棒在细胞膜上的扩散运动将单颗粒示踪技术引入本科生教学，并将其作为化学测量学的示范性试验。本实验内容丰富、趣味性强，面向已基本掌握化学、化学生物学等基础知识并对化学测量学感兴趣的二、三年级本科生，旨在将单颗粒示踪领域研究前沿融入本科生教学，引导学生自主探索，有利于激发学生的科研兴趣与培养专业素养。

关键词: 单颗粒示踪, 等离激元纳米颗粒, 活细胞成像, 科教融合, 综合化学实验

Abstract: Single-particle tracking represents a significant technological breakthrough for real-time observation of dynamic biological processes, particularly in uncovering mechanisms hidden in static observations. With advancements in optical microscopy, commercial instruments now enable real-time observation of biological processes at the microscopic scale. This experiment explores the size effect of plasmonic nanomaterials using gold and silver nanoparticles as imaging probes. Additionally, live cell imaging experiments are designed to introduce single-particle tracking into undergraduate education by observing the diffusion of gold nanorods on cell membranes as an exemplary experiment in Chemical Metrology and Technology. The experiment is rich in content and engaging, targeting second- and third-year undergraduates who have a foundational understanding of chemistry and chemical biology and are interested in Chemical Metrology and Technology. The goal is to integrate cutting-edge research in single-particle tracking into undergraduate teaching, encouraging independent exploration and stimulating students’ interest in scientific research while cultivating their professional skills.

Key words: Single-particle tracking, Plasmonic nanoparticles, Live-cell imaging, Integration of science and education, Integrated chemistry experiments