大学化学 >> 2026, Vol. 41 >> Issue (5): 285-293.doi: 10.12461/PKU.DXHX202509114

所属专题： 第5届全国大学生化学实验创新设计大赛

专题 上一篇    

共轭聚合物探针的设计及其对藻蓝蛋白的智能检测

张军杰1, 董超超1, 李锦科1, 郭嘉文1, 水英鑫1, 王悦1,2, 段新华1   

  1. 1 西安交通大学化学学院, 陕西 西安 710049;
    2 西安交通大学医学部药学院, 陕西 西安 710061
  • 收稿日期:2025-09-24 录用日期:2026-01-30 发布日期:2026-05-21
  • 通讯作者: 张军杰, 段新华 E-mail:junjiezhang@xjtu.edu.cn;duanxh@mail.xjtu.edu.cn Junjie Zhang, Xinhua Duan
  • 基金资助:
    教育部产学合作协同育人项目(241003242182935)；西安交通大学本科教学改革研究项目(2405Y)；陕西省自然科学基础研究计划面上项目(2024JC-YBMS-133)

Design of conjugated polymer probes for intelligent detection of phycocyanin

Junjie Zhang1, Chaochao Dong1, Jinke Li1, Jiawen Guo1, Yingxin Shui1, Yue Wang1,2, Xinhua Duan1   

  1. 1 School of Chemistry, Xi'an Jiaotong University, Xi'an 710049, Shaanxi Province, China;
    2 School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China
  • Received:2025-09-24 Accepted:2026-01-30 Published:2026-05-21
  • Contact: Junjie Zhang, Xinhua Duan E-mail:junjiezhang@xjtu.edu.cn;duanxh@mail.xjtu.edu.cn

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摘要： 本实验关注蓝藻水华早期预警的生态安全问题，创新性地将共轭聚合物荧光探针对蓝藻水华中藻蓝蛋白的检测转化为12学时的本科生综合实验，包含探针合成、仪器表征、方法建立和样品检测四个模块，融合有机化学、高分子化学、分析化学、仪器分析等多学科知识。自主研发的便携式智能荧光传感平台，显著提升实验教学的便捷性和学生的参与度，促进学生实验技能、数据分析能力、创新思维和科研水平的协同发展，全面提升学生的综合实验素养。该实验具有成本低、操作简便、安全性高等特点，适合在化学及相关专业高年级本科生的“综合化学实验”中开设。该荧光探针的设计思路具有普适性，可拓展应用于不同领域的目标分析物检测；智能荧光传感平台兼具高通用性与低成本优势，适用于各类基于荧光分析的实验教学，具备良好的推广应用价值。

关键词: 藻蓝蛋白, 共轭聚合物, 智能检测, 综合实验

Abstract: This study addresses ecological security concerns through early warning of cyanobacterial blooms by developing an innovative 12-hour comprehensive undergraduate experiment. The curriculum integrates conjugated polymer fluorescent probes for phycocyanin detection into four modules: probe synthesis, instrumental characterization, method development, and sample analysis, incorporating multidisciplinary knowledge from organic chemistry, polymer chemistry, analytical chemistry, and instrumental analysis. Our self-developed portable intelligent fluorescence sensing platform significantly enhances teaching efficiency and student engagement while promoting the coordinated development of experimental skills, data analysis capabilities, innovative thinking, and research competence. Characterized by low cost, operational simplicity, and high safety, this experiment effectively bridges fundamental techniques with advanced understanding of structure-property-application relationships, making it ideal for upper-level chemistry majors in “Comprehensive Chemical Experiment” courses. The probe design demonstrates universal applicability for various target analytes, and the versatile, cost-effective sensing platform shows excellent potential for widespread adoption in fluorescence-based experimental teaching.

Key words: Phycocyanin, Conjugated polymer, Intelligent detection, Comprehensive experiment