大学化学 >> 2022, Vol. 37 >> Issue (5): 2111071.doi: 10.3866/PKU.DXHX202111071

所属专题： 第2届全国大学生化学实验创新设计竞赛

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基于超分子自组装策略定性和定量检测奶粉中的三聚氰胺

察爽爽, 牛金盆, 刘川枫, 严杰, 朱敏, 张雯, 胡明友()   

  • 收稿日期:2021-11-24 录用日期:2021-12-07 发布日期:2022-04-06
  • 通讯作者: 胡明友 E-mail:mingyouhu@xjtu.edu.cn
  • 作者简介:胡明友, Email: mingyouhu@xjtu.edu.cn
    第一联系人：

    †共同第一作者，对本文工作同等贡献

  • 基金资助:
    西安交通大学本科实践教学改革研究专项项目(20SJZX34);国家自然科学基金(21901196);陕西省自然科学基金(2020JQ-016)

Qualitative and Quantitative Determination of Melamine in Milk Powder Based on Supramolecular Self-Assembly Strategy

Shuangshuang Cha, Jinpen Niu, Chuanfeng Liu, Jie Yan, Min Zhu, Wen Zhang, Mingyou Hu()   

  • Received:2021-11-24 Accepted:2021-12-07 Published:2022-04-06
  • Contact: Mingyou Hu E-mail:mingyouhu@xjtu.edu.cn

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摘要：

三聚氰胺是一种应用广泛的化工原料，但在摄入人体后会形成结石，对肾功能造成极大损伤。由于其含氮量高，常被不法商家添加在奶粉中以提高奶粉含氮量，因此在食品工业中三聚氰胺的定性定量检测十分重要。本研究利用超分子自组装的策略，以三聚氰胺与氰尿酸自组装作用为基础，利用亚甲基蓝作为反应指示剂，使用操作便捷的分光光度法对奶粉中的三聚氰胺进行定性和定量检测。本方法反应条件温和、颜色变化明显、检出限低、灵敏度高，也可拆分成多个环节实验，应用于不同学时的实验教学，是一个适合本科教学环境的新颖实验。

关键词: 三聚氰胺, 超分子自组装, 吸光度, 指示剂

Abstract:

Melamine is a widely used industrial raw material that forms stones after ingestion, causing great damage to the kidneys. Because of its high nitrogen content, melamine is often illegally added to milk powder to improve the nitrogen content. Therefore, the qualitative and quantitative detection of melamine in the food industry is critical. This study was based on the supramolecular self-assembly strategy; cyanuric acid was selected as the detection reagent to form the supramolecular structure with melamine, and methylene blue was used as an indicator through the interaction of melamine and cyanuric acid. The melamine in milk powder was quantitatively detected by an ultraviolet spectrophotometer. The reaction conditions of this experiment were mild, the color change was clear, the detection limit was low, and the sensitivity was high. The experiment can be divided into many experimental units and is a novel experiment, thus making it suitable for the undergraduate teaching.

Key words: Melamine, Supramolecular self-assembly, Absorbance, Indicator