大学化学 >> 2023, Vol. 38 >> Issue (9): 196-201.doi: 10.3866/PKU.DXHX202211056

化学实验 上一篇    下一篇

紫外-荧光检测器串联高效液相色谱法测定维生素B2片含量的实验设计

谢一凡1,2, 姚莉韵1,2,*(), 杨若林2, 蔡玉兴1, 金玉杰1, 李宁1   

  1. 1 上海交通大学医学院, 基础医学实验教学中心, 上海 200025
    2 上海交通大学医学院, 药物化学与生物信息学中心, 上海 200025
  • 收稿日期:2022-11-23 录用日期:2023-01-03 发布日期:2023-09-21
  • 通讯作者: 姚莉韵 E-mail:yaoliyun517@163.com
  • 基金资助:
    上海交通大学医学院课程建设基金项目(BJ13000180095)

Experimental Design for HPLC Determination of Vitamin B2 in Tablets via UVD-FLD Connected in Series

Yifan Xie1,2, Liyun Yao1,2,*(), Ruolin Yang2, Yuxing Cai1, Yujie Jin1, Ning Li1   

  1. 1 Basic Medical Experimental Teaching Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
    2 Medical Chemistry and Bioinformatics Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
  • Received:2022-11-23 Accepted:2023-01-03 Published:2023-09-21
  • Contact: Liyun Yao E-mail:yaoliyun517@163.com

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摘要：

设计一种紫外检测器(UVD)与荧光检测器(FLD)串联的高效液相色谱法(HPLC)测定维生素B2片含量的方法，并应用于实验教学。该实验方案能克服常规HPLC法(单一检测器)存在的缺限，同时实验过程中学生不仅掌握了HPLC的操作，而且能了解UVD和FLD的原理及应用，利于培养学生的综合检测能力和拓展思维能力。

关键词: 高效液相色谱法, 紫外-荧光双检测器, 维生素B2片, 含量测定, 实验教学

Abstract:

The high performance liquid chromatography (HPLC) method of UV detector-fluorescence detector (UVD-FLD) connected in series for the determination of vitamin B2 in tablets was designed as a teaching experiment. This method can overcome the limitations of common HPLC by a single detector. Through the application of this method for experimental teaching, students could not only master the basic theory and operation of HPLC, but also understand the principles and functions of UVD and FLD. Furthermore, it also improved the undergraduate students' experimental skill and comprehension.

Key words: HPLC, UV detector-fluorescence detector, Vitamin B2, Content determination, Laboratory teaching