固氮酶活性中心研究五十年

doi:10.3866/PKU.DXHX202310133

大学化学 >> 2024, Vol. 39 >> Issue (7): 287-293.doi: 10.3866/PKU.DXHX202310133

固氮酶活性中心研究五十年

陈全亮¹, 周朝晖²

1 西南民族大学化学与环境学院, 成都 610041;
2 厦门大学化学化工学院, 固体表面物理化学国家重点实验室, 福建厦门 361005

收稿日期:2023-10-31 录用日期:2024-02-01 发布日期:2024-02-21
通讯作者: 陈全亮 E-mail:qlchen@swun.edu.cn
基金资助:
国家自然科学基金(22179110)

Research on the Active Site of Nitrogenase over Fifty Years

Quanliang Chen¹, Zhaohui Zhou²

1 College of Chemistry and Environment, Southwest Minzu University, Chengdu 610041, China;
2 State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian Province, China

Received:2023-10-31 Accepted:2024-02-01 Published:2024-02-21
Contact: Quanliang Chen E-mail:qlchen@swun.edu.cn

摘要/Abstract

摘要： 固氮酶是固氮微生物在常温常压下固氮成氨的催化剂，其活性中心的结构从Fe₂S₂∙Mo₂O₂演变到MoFe₇S₉C(R-Hhomocit)(cys)(his) (H₄homocit = 高柠檬酸，Hcys = 半胱氨酸，Hhis = 组氨酸)。本文将回顾这一重要的历史过程，探讨化学模拟、光谱学、理论计算和生物化学，特别是结构生物学等领域在固氮酶活性中心研究中的重要成就，从化学结构的角度看固氮酶活性中心研究五十年。

关键词: 固氮酶, 钼铁蛋白, 铁钼辅基, 活性中心

Abstract: Nitrogenase is a catalyst used by nitrogen-fixing microorganisms to convert atmospheric nitrogen into ammonia at ambient temperature and pressure. The structure of the active site in molybdenum nitrogenase has evolved from Fe₂S₂∙Mo₂O₂ to MoFe₇S₉C(R-Hhomocit)(cys)(his) (H₄homocit = homocitric acid, Hcys = cysteine, Hhis = histidine) through advancements in chemical modeling, spectroscopy, and theoretical calculations, especially for structural biology. This paper provides a comprehensive review of the important achievements in the study of the active site of nitrogenase from a chemical structure perspective over the past fifty years.

Key words: Nitrogenase, MoFe-protein, FeMo-cofactor, Active site

陈全亮, 周朝晖. 固氮酶活性中心研究五十年[J]. 大学化学, 2024, 39(7): 287-293.

Quanliang Chen, Zhaohui Zhou. Research on the Active Site of Nitrogenase over Fifty Years[J]. University Chemistry, 2024, 39(7): 287-293.

链接本文: https://www.dxhx.pku.edu.cn/CN/10.3866/PKU.DXHX202310133

https://www.dxhx.pku.edu.cn/CN/Y2024/V39/I7/287

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固氮酶活性中心研究五十年

Research on the Active Site of Nitrogenase over Fifty Years

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