大学化学 >> 2023, Vol. 38 >> Issue (12): 212-219.doi: 10.3866/PKU.DXHX202304094

化学实验 上一篇    下一篇

智能型药物控释可视化系统的构建与表征——高分子实验的新设计与教学实践

张来英(), 苟洪瑀, 庄海妮, 秦宇航, 邱惠娟, 吴伟泰()   

  • 收稿日期:2023-04-26 录用日期:2023-06-21 发布日期:2023-06-29
  • 通讯作者: 张来英,吴伟泰 E-mail:wuzhly@xmu.edu.cn;wuwtxmu@xmu.edu.cn
  • 基金资助:
    2021年度教育部“基础学科拔尖学生培养计划2.0”研究课题;国家自然科学基金(21574107);国家自然科学基金(21774105);2020年度福建省“雏鹰计划”青年拔尖人才支持项目

Construction and Characterization of Intelligent, Controlled Drug Release Visualization Systems: A New Design and Teaching Practice of Polymer Experiments

Laiying Zhang(), Hongyu Gou, Haini Zhuang, Yuhang Qin, Huijuan Qiu, Weitai Wu()   

  • Received:2023-04-26 Accepted:2023-06-21 Published:2023-06-29
  • Contact: Laiying Zhang, Weitai Wu E-mail:wuzhly@xmu.edu.cn;wuwtxmu@xmu.edu.cn

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摘要：

介绍一个涵盖高分子化学、物理化学与仪器分析等领域的实验新设计与教学实践。通过水相自由基沉淀聚合法合成一种温敏性微凝胶，并使用紫外-可见分光光度计和动态光散射仪对其温度响应特性进行表征，进而将其用作载体材料以设计构建智能型药物控释系统，利用微凝胶低温溶胀、高温收缩的特性，实现药物的装载、释放。提出结合色彩理论与化学知识来理解药物释放过程，以在可见光区存在明显吸收的药物作为模型药物，将“没有明显实验现象”的药物释放过程“可视化”，以更加直观的方式观测药物释放过程，并在此“可视化”感性认识的基础上，理性分析药物释放过程的分子扩散机理。本实验取材于广受关注且已有较多验证的研究前沿成果，绿色环保，并且可拓展至其他响应性高分子及药物，具有普适性。本实验主要目标是在锻炼学生基本操作能力的同时，培养他们发现问题、解决问题的研究技能，提升学生创新能力。

关键词: 温敏性微凝胶, 自由基沉淀聚合, 药物控释系统, 可视化检测, 实验教学

Abstract:

This study presents a new experimental design and teaching practice for polymer chemistry, physical chemistry, and instrument analysis. Here, a type of thermosensitive polymer microgel was synthesized in an aqueous solution by free radical precipitation polymerization. The thermo-responsivity of these microgels was assessed using a UV-Vis spectrophotometer and dynamic light scattering instrument. These microgels were then utilized as carriers to construct a controlled drug delivery system, in which the drug was uploaded to the microgels in the swollen state and released from the microgels in the collapsed state. To understand the drug release process, a combination of color theory and chemical knowledge was proposed, in which the drug with visible range absorption was selected to "visualize" the drug release process, making it more intuitive and observable. Based on this perceptual understanding, the molecular diffusion mechanism of the drug release process was analyzed rationally. This experiment was based on cutting-edge green research results that have been widely investigated and verified. Moreover, this experiment, which can be extended to other responsive polymers and drugs, has universal applicability. This experiment can not only exercise basic operational ability but also help develop research skills for identifying and solving problems and improve students' innovation ability.

Key words: Thermo-sensitive microgels, Free radical precipitation polymerization, Controlled drug release system, Visual detection, Experimental teaching