大学化学 >> 2025, Vol. 40 >> Issue (6): 201-209.doi: 10.12461/PKU.DXHX202408070

化学实验 上一篇    下一篇

一种基于香豆素氟离子荧光探针的合成、表征及性能测试——“科研反哺教学”在有机化学综合实验教学中的探索与实践

王本花, 姚朝怡, 李一鸣, 刘清, 蓝敏焕, 喻桂朋, 罗一鸣, 宋相志   

  1. 中南大学化学化工学院, 长沙 410083
  • 收稿日期:2024-08-15 录用日期:2024-09-25 发布日期:2025-06-20
  • 通讯作者: 宋相志 E-mail:xzsong@csu.edu.cn
  • 基金资助:
    湖南省教育厅一般项目(2023jy134)

Synthesis, Characterization, and Performance Evaluation of a Coumarin-based Fluorescent Probe for Fluoride Ions: An Exploration of “Research Feedback Teaching” in Organic Chemistry Comprehensive Experiment

Benhua Wang, Chaoyi Yao, Yiming Li, Qing Liu, Minhuan Lan, Guipeng Yu, Yiming Luo, Xiangzhi Song   

  1. College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
  • Received:2024-08-15 Accepted:2024-09-25 Published:2025-06-20
  • Contact: SONG Xiangzhi E-mail:xzsong@csu.edu.cn

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摘要： 基于科研实验，深入融合有机化学基本理论和实验技术，开发了一项研究型有机化学综合实验。该实验以4-(二乙氨基)水杨醛为原料，通过亲核取代和Knoevenagel缩合反应，合成了一种基于香豆素荧光团的氟离子荧光探针。利用柱层析纯化产物，采用核磁共振技术对产物进行结构表征，最终使用紫外-可见光谱和荧光光谱分析了该探针对氟离子的检测性能。通过本实验，学生不仅加深了对有机化学反应基本原理的学习、牢固掌握了有机化学基本实验操作，而且深入了解有机荧光染料在分子检测和识别中的应用，实现了理论知识与实际应用的有机融合。本实验的“科研反哺教学”模式，有效地将科研创新与教学实践相融合，极大激发了学生学习理论知识的积极性和参与科研实践的热情，培养了学生们的科研素养和创新能力，实验教学效果显著。

关键词: 科研反哺教学, 香豆素荧光探针, 氟离子, 有机化学

Abstract: This study describes a research-oriented organic chemistry experiment that deeply integrates fundamental theories and experimental techniques of organic chemistry. Utilizing 4-(diethylamino)salicylaldehyde as the starting material, a coumarin-based fluorescent probe for fluoride ions was synthesized through nucleophilic substitution and Knoevenagel condensation reactions. The product was purified using column chromatography and characterized via nuclear magnetic resonance (NMR) spectroscopy. The probe’s performance in detecting fluoride ions was evaluated using UV-Vis and fluorescence spectroscopy. This experiment not only deepens students’ comprehension of the fundamental principles of organic reactions and solidifies their basic experimental skills but also enhances their understanding of the application of organic fluorescent dyes in molecular detection. It effectively merges theoretical knowledge with practical application. The “research feedback teaching” model successfully integrates scientific innovation with educational practice, significantly motivating students to engage with theoretical concepts and participate in scientific research, thereby cultivating their scientific literacy and innovative capabilities. The outcomes of this experimental teaching approach have been outstanding.

Key words: Research feedback teaching, Coumarin-based fluorescent probe, Fluoride ion, Organic chemistry