大学化学 >> 2025, Vol. 40 >> Issue (10): 194-202.doi: 10.12461/PKU.DXHX202411063

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研究型仪器分析综合实验课程的案例与实践

吴倩, 吕远霞, 郭紫旋, 赵治皓, 张志敏, 卢红梅   

  1. 中南大学化学化工学院, 长沙 410083
  • 收稿日期:2024-11-19 录用日期:2025-01-20 发布日期:2025-09-17
  • 通讯作者: 吴倩 E-mail:qianwu@csu.edu.cn
  • 基金资助:
    湖南省教学改革研究项目(HNJG-20230117); 国家自然科学基金面上项目(22474156, 22074159); 国家自然科学基金青年项目(21804142);湖南省自然科学基金优青项目(2022JJ20054)

A Case Study and Practice of Research-Oriented Comprehensive Instrumental Analysis Laboratory Courses

Qian Wu, Yuanxia Lv, Zixuan Guo, Zhihao Zhao, Zhimin Zhang, Hongmei Lu   

  1. College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
  • Received:2024-11-19 Accepted:2025-01-20 Published:2025-09-17
  • Contact: Qian Wu E-mail:qianwu@csu.edu.cn

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摘要： 本文设计了一个以科学研究为背景，结合多种仪器分析方法的综合仪器分析实验。此实验以代谢组学为背景，由气相色谱-质谱与液相色谱-质谱联合表征血浆中小分子。一个实验掌握三种分析仪器的原理和操作，解决课程缺乏知识关联性的问题。通过仪器结构的深度讲解和开放实验参数设置的方式提高实验探索性，调动学生主观能动性。

关键词: 仪器分析实验课, 研究型课程, 色谱-质谱联用, 代谢组学, 血浆

Abstract: This study presents a research-oriented comprehensive instrumental analysis laboratory course designed within a scientific research context. We developed an integrated experiment combining gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) for metabolomic profiling of plasma small molecules. This approach enables students to master the principles and operational techniques of three analytical instruments (GC, LC, and MS) through a single experiment, effectively addressing the issue of knowledge fragmentation in traditional courses. By incorporating detailed instrument structure explanations and open experimental parameter settings, we significantly enhanced the course’s exploratory nature and actively engaged students' initiative in learning.

Key words: Instrumental analysis laboratory class, Research-oriented courses, Chromatography coupled with mass spectrometry, Metabolomics, Blood plasma