大学化学 >> 2024, Vol. 39 >> Issue (4): 395-400.doi: 10.3866/PKU.DXHX202310047

自学之友 上一篇    

基于米氏机理的酶催化反应中反应速率常数的计算

张恒   

  1. 武汉大学化学与分子科学学院, 武汉 430072
  • 收稿日期:2023-10-13 修回日期:2023-11-28 发布日期:2024-04-17
  • 通讯作者: 张恒 E-mail:hengzhang@whu.edu.cn

Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism

Heng Zhang   

  1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China
  • Received:2023-10-13 Revised:2023-11-28 Published:2024-04-17

PDF

694

赞

摘要： 对基于米氏机理的酶催化反应模型，本文介绍了求解全部反应速率常数的两种方法：瞬态法和数学计算法，其中瞬态法的核心是分析反应达到稳态前的过程，数学计算法的核心是寻找反应过程中酶与底物浓度之间的关系。通过求解全部反应速率常数，不仅可以加深对酶催化反应动力学的理解，而且有助于提高对反应动力学的认识。

关键词: 酶催化反应, 动力学, 米氏机理, 速率常数

Abstract: Two protocols, the transient method and the mathematical method, are introduced to deduce all the rate constants in the enzyme-catalyzed reaction model based on the Michaelis-Menten mechanism. The transient method focuses on analyzing the period before reaching a steady state, while the mathematical method aims to establish a relationship between the concentration of the enzyme and the substrate. By calculating all the rate constants, one can enhance their understanding and grasp of the kinetics of the enzyme-catalyzed reaction.

Key words: Enzyme catalyzed reaction, Kinetics, Michaelis-Menten mechanism, Rate constant